Che Cheng-Ye, Li Cui, Gao Ang, Lin Jing, Zhang Li-Li, Xu Qiang, Wang Qian, Zhao Gui-Qiu
Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
Int J Ophthalmol. 2013;6(1):30-3. doi: 10.3980/j.issn.2222-3959.2013.01.06. Epub 2013 Feb 18.
To investigate the expression of dendritic cell-associated C-type lectin-1(dectin-1) at the early period of Aspergillus fumigatus infection in rat's corneal epithelium.
A total of 72 Wistar rats were randomly divided into three groups: A, B and C. The right eyes were chosen as experimental eyes. Group A was control group. Rats in group B were not inoculated with Aspergillus fumigatus. Group C was taken as Aspergillus fumigatus keratitis model. Rats in group B and C (six from each group) were executed randomly at 4, 8, 16 and 24 hours after experimental model being established to assess the expression of dectin-1 mRNA through real-time PCR. Another six rats in group B and C were executed randomly at 24 hours to assess the expression of dectin-1 protein through immunohistochemistry.
The results of real-time PCR indicated that dectin-1 mRNA expression was low in corneal epithelium of normal rats'. There was no significantly difference of dectin-1 mRNA expression in group A and B (P>0.05). The expression of Aspergillus fumigatus infected corneal epithelium increased gradually after 8 hours in group C. The synchronous expression of group A and C had significant difference (P<0.01). Immunohistochemisty discovered that dectin-1 receptor existed in normal rat's corneal epithelium. Dectin-1 protein increased after 24 hours in group C. There was a significant difference of synchronous expression in group B and C (P<0.01).
Dectin-1 exists in rat's corneal epithelium and its expression significantly increases at the early period of Aspergillus fumigatus infection. Dectin-1 is a pattern recognition receptor that expresses in corneal epithelium and involves in immune response to Aspergillus fungal keratitis.
研究树突状细胞相关C型凝集素-1（dectin-1）在烟曲霉感染大鼠角膜上皮早期的表达情况。
将72只Wistar大鼠随机分为A、B、C三组。选取右眼作为实验眼。A组为对照组。B组大鼠不接种烟曲霉。C组作为烟曲霉角膜炎模型组。在建立实验模型后4、8、16和24小时，随机处死B组和C组（每组6只）大鼠，通过实时荧光定量PCR评估dectin-1 mRNA的表达。在2种24小时，随机处死B组和C组另外6只大鼠，通过免疫组织化学评估dectin-1蛋白的表达。
实时荧光定量PCR结果显示，正常大鼠角膜上皮中dectin-1 mRNA表达较低。A组和B组dectin-1 mRNA表达无显著差异（P>0.05）。C组烟曲霉感染角膜上皮在8小时后表达逐渐增加。A组和C组的同步表达有显著差异（P<0.01）。免疫组织化学发现，正常大鼠角膜上皮中存在dectin-1受体。C组24小时后dectin-1蛋白增加。B组和C组的同步表达有显著差异（P<0.01）。
Dectin-1存在于大鼠角膜上皮中，在烟曲霉感染早期其表达显著增加。Dectin-1是一种在角膜上皮中表达的模式识别受体，参与对烟曲霉性真菌性角膜炎的免疫反应。
