Mao Jun-Feng, Liu Shuang-Zhen, Qin Wen-Juan, Xiang Qian
Department of Ophthalmology, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China.
Int J Ophthalmol. 2011;4(4):357-60. doi: 10.3980/j.issn.2222-3959.2011.04.06. Epub 2011 Aug 18.
To investigate the effect of protein kinase C (PKC) on transforming growth factor-β(2) (TGFβ(2)) and dopamine in retinal Müller cells of guinea pig myopic eye.
Myopia was induced by translucent goggles in guinea pig, whose retinal Müller cells were cultured using the enzyme-digesting method. Retinal Müller cells were divided into 5 groups: normal control, myopia, myopia plus GF109203X, myopia plus PMA, myopia plus DMSO. PKC activities were detected by the non-radioactive methods. TGFβ(2) and tyrosine hydroxylase (TH) proteins were analyzed by Western Blotting in retinal Müller cells. Dopamine was determined by the high-performance liquid chromatography-electrochemical detection in suspensions.
After 14 days deprived, the occluded eyes became myopic with ocular axle elongating. Müller cells of guinea pigs were obtained using enzyme digestion. Compared with normal control group, the increase in PKC activity and the up-regulation in TGFβ(2) expression were found in retinal Müller cells of myopic eyes, with the decrease of TH and dopamine content (P<0.05). After PKC activated by PMA, TGFβ(2) and TH content were up-regulated with the increase of dopamine content (P<0.05). While the PKC activities was inhibited by GF109203X, proteins of TGFβ(2) and TH were down-regulated in the myopic eyes, with the decrease of dopamine content (P<0.05).
TGFβ(2) and dopamine are modulated by PKC in Müller cells of the myopic eyes in guinea pig.
研究蛋白激酶C（PKC）对豚鼠近视眼视网膜Müller细胞中转化生长因子-β2（TGFβ2）和多巴胺的影响。
用半透明眼罩诱导豚鼠近视，采用酶消化法培养其视网膜Müller细胞。将视网膜Müller细胞分为5组：正常对照组、近视组、近视+GF109203X组、近视+佛波酯（PMA）组、近视+二甲基亚砜（DMSO）组。采用非放射性方法检测PKC活性。通过蛋白质免疫印迹法分析视网膜Müller细胞中TGFβ2和酪氨酸羟化酶（TH）蛋白。采用高效液相色谱-电化学检测法测定悬液中的多巴胺。
剥夺14天后，遮盖眼出现近视，眼轴延长。采用酶消化法获得豚鼠Müller细胞。与正常对照组相比，近视眼视网膜Müller细胞中PKC活性增加，TGFβ2表达上调，TH和多巴胺含量降低（P<0.05）。PMA激活PKC后，TGFβ2和TH含量上调，多巴胺含量增加（P<0.05）。而GF109203X抑制PKC活性后，近视眼TGFβ2和TH蛋白下调，多巴胺含量降低（P<0.05）。
在豚鼠近视眼的Müller细胞中，TGFβ2和多巴胺受PKC调节。
