大豆分离蛋白喂养对 DOCA 盐处理肥胖 Zucker 大鼠严重肾损伤的影响。

Effects of soy protein isolate feeding on severe kidney damage in DOCA salt-treated obese Zucker rats.

机构信息

Nutrition and Health Department, Food Science Research Institute, Fuji Oil Company Ltd., Izumisano-shi, Osaka 598-8540, Japan.

出版信息

J Agric Food Chem. 2012 May 30;60(21):5367-72. doi: 10.1021/jf300598a. Epub 2012 May 16.

DOI:10.1021/jf300598a

PMID:22553937

Abstract

This study assessed the effects of soy protein isolate (SPI) on severe kidney damage in deoxycorticosterone acetate (DOCA) salt-treated obese Zucker rats. These rats underwent heminephrectomy and were fed either casein or SPI diet for 12 weeks. From weeks 8 to 10 of the experiment, kidney damage was induced by biweekly injection of 25 mg/kg DOCA and administration of 0.5% NaCl (w/v) ad libitum. Urinary protein and N-acetyl-β-d-glucosaminidase excretions of SPI rats were much lower than those of casein rats at weeks 1 (p < 0.01) and 2 (p < 0.05) after DOCA treatment. Abnormal mineral excretions induced by DOCA treatment in casein rats were hardly detected in SPI rats. Severe atrophy of tubular epithelium and some flattened/detached renal tubules were also observed in casein rats, but not in SPI rats. These results indicate that consecutive treatment of SPI protects against renal dysfunction, particularly tubulointerstitial nephritis, in DOCA salt-treated obese Zucker rats.

摘要

本研究评估了大豆分离蛋白（SPI）对去氧皮质酮醋酸盐（DOCA）盐处理肥胖 Zucker 大鼠严重肾损伤的影响。这些大鼠接受了半肾切除术，并在 12 周内分别喂食酪蛋白或 SPI 饮食。在实验的第 8 至 10 周，通过每两周注射 25mg/kg DOCA 和自由给予 0.5%NaCl（w/v）诱导肾脏损伤。与酪蛋白组大鼠相比，SPI 组大鼠在 DOCA 处理后第 1 周（p<0.01）和第 2 周（p<0.05）的尿蛋白和 N-乙酰-β-D-氨基葡萄糖苷酶排泄量明显更低。在酪蛋白组大鼠中，DOCA 处理引起的异常矿物质排泄在 SPI 组大鼠中几乎未被检测到。在酪蛋白组大鼠中还观察到管状上皮严重萎缩和一些扁平/分离的肾小管，但在 SPI 组大鼠中没有观察到。这些结果表明，连续 SPI 治疗可预防 DOCA 盐处理肥胖 Zucker 大鼠的肾功能障碍，特别是肾小管间质性肾炎。