Ohta Satoshi, Asanoma Masashi, Irie Nao, Tachibana Nobuhiko, Kohno Mitsutaka
Research Institute for Creating the Future, Fuji Oil Holdings Inc., 4-3 Kinunodai, Tsukubamirai-shi 300-2497, Ibaraki, Japan.
Soy Ingredients R&D Department, Fuji Oil Co., Ltd., 1 Sumiyoshicho, Izumisano-shi 598-8540, Osaka, Japan.
Metabolites. 2022 Apr 6;12(4):330. doi: 10.3390/metabo12040330.
Complications associated with chronic kidney disease (CKD), which involves kidney inflammation, are a major health problem. Soy protein isolate (SPI) reportedly inhibits CKD exacerbation; however, its detailed action mechanism remains obscure. Therefore, the role of the polar lipid component of SPI in suppressing inflammation was investigated. Zucker fatty rats were divided into three groups and fed a diet containing casein, SPI, or casein + SPI ethanol extract (SPIEE) for 16 weeks. The isoflavones and phospholipids of SPIEE were evaluated for their anti-inflammatory effects. Rats in the SPI and casein + SPIEE groups showed reduced levels of the urinary -acetyl-β-d-glucosaminidase and renal IL-1β mRNA (an inflammatory marker) compared with those in the casein group. In proximal tubular cells, genistein significantly inhibited monocyte chemoattractant protein-1 (MCP-1) expression induced by an IL-1β stimulus. In macrophages, soybean phospholipids suppressed lipopolysaccharide-induced IL-1β gene expression by inhibiting the phosphorylation of inhibitor κB and p65. Phosphatidylinositol (PI) was found to be essential for inhibition of IL-1β expression. SPIEE inhibited the exacerbation of kidney disease. Genistein and soybean phospholipids, especially soybean-specific phospholipids containing PI, effectively inhibited the inflammatory spiral in vitro. Hence, daily soybean intake may be effective for inhibiting chronic inflammation and slowing kidney disease progression.
与涉及肾脏炎症的慢性肾脏病（CKD）相关的并发症是一个主要的健康问题。据报道，大豆分离蛋白（SPI）可抑制CKD的恶化；然而，其详细的作用机制仍不清楚。因此，研究了SPI的极性脂质成分在抑制炎症中的作用。将 Zucker 肥胖大鼠分为三组，分别喂食含酪蛋白、SPI 或酪蛋白+SPI 乙醇提取物（SPIEE）的饮食 16 周。评估了 SPIEE 中的异黄酮和磷脂的抗炎作用。与酪蛋白组相比，SPI 组和酪蛋白+SPIEE 组大鼠的尿β-乙酰基-β-D-氨基葡萄糖苷酶水平和肾脏 IL-1β mRNA（一种炎症标志物）水平降低。在近端肾小管细胞中，染料木黄酮显著抑制 IL-1β刺激诱导的单核细胞趋化蛋白-1（MCP-1）表达。在巨噬细胞中，大豆磷脂通过抑制抑制剂κB和p65的磷酸化来抑制脂多糖诱导的IL-1β基因表达。发现磷脂酰肌醇（PI）对于抑制IL-1β表达至关重要。SPIEE 抑制了肾脏疾病的恶化。染料木黄酮和大豆磷脂，尤其是含有 PI 的大豆特异性磷脂，在体外有效抑制了炎症螺旋。因此，每日摄入大豆可能对抑制慢性炎症和减缓肾脏疾病进展有效。
