Li Jie, Zhang Ping, Wang Peiyuan, Alsarakibi Muhamd, Zhu Haibo, Liu Yuanjia, Meng Xianglong, Li Jinping, Guo Jianchao, Li Guoqing
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, Guangdong Province 510642, People's Republic of China.
Vet Parasitol. 2012 Sep 10;188(3-4):368-71. doi: 10.1016/j.vetpar.2012.04.004. Epub 2012 Apr 5.
Giardia duodenalis is a flagellated parasite and is considered one of the most common causes of protozoal diarrhea in both humans and animals worldwide. This paper represents the first study of the prevalence of G. duodenalis in pet dogs in Guangzhou, China. Faecal samples (209 specimens) were obtained from young (<6 months old), adult (6 months to 3 years) and elder dogs (>3 years old). 8.61% (18/209) faecal samples were recorded positive using microscopy examination, and 11.00% (23/209) using PCR. The prevalence was significantly higher in diarrheic dogs (26.31%) compared with non-diarrheic dogs (5.10%), while it was higher in young (25.58%) than both adult (7.37%) and elder (7.04%) dogs and the difference was statistically significant (P<0.05). The prevalence in male dogs 11.30% (13/115) was higher than females 10.87% (10/92), and in suburban dogs (12.15%) higher than urban 9.80%, but the difference was not statistically significant (P>0.05). Sequence analysis of the 23 PCR-positive samples revealed the presence of Assemblage D (18/23), and zoonotic Assemblage A (5/23). The present investigation reported a high infection rate of G. duodenalis in pet dogs, especially in young dogs. Genotypic characterization demonstrated that the zoonotic Assemblage A was found, a fact that poses a potential risk of G. duodenalis transmission from pet dogs to humans. It is suggested that pet owners should take appropriate hygiene measures to prevent and control giardiasis in this region.
十二指肠贾第虫是一种有鞭毛的寄生虫，被认为是全球人类和动物原生动物腹泻最常见的病因之一。本文是对中国广州宠物狗中十二指肠贾第虫感染率的首次研究。粪便样本（209份标本）取自幼犬（<6个月大）、成年犬（6个月至3岁）和老年犬（>3岁）。显微镜检查记录8.61%（18/209）的粪便样本呈阳性，PCR检测为11.00%（23/209）。腹泻犬的感染率（26.31%）显著高于非腹泻犬（5.10%），而幼犬的感染率（25.58%）高于成年犬（7.37%）和老年犬（7.04%），差异具有统计学意义（P<0.05）。公犬的感染率为11.30%（13/115），高于母犬的10.87%（10/92），郊区犬的感染率（12.15%）高于城区犬的9.80%，但差异无统计学意义（P>0.05）。对23份PCR阳性样本的序列分析显示存在D组（18/23）和人畜共患的A组（5/23）。本调查报道宠物狗中十二指肠贾第虫的感染率很高，尤其是幼犬。基因型特征表明发现了人畜共患的A组，这一事实构成了十二指肠贾第虫从宠物狗传播给人类的潜在风险。建议宠物主人采取适当卫生措施，预防和控制该地区的贾第虫病。
