The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Parasit Vectors. 2018 Mar 20;11(1):199. doi: 10.1186/s13071-018-2790-z.
Giardia duodenalis is a flagellated parasite that causes diarrhea in humans and other animals. Although G. duodenalis is found in companion animals worldwide, information regarding the prevalence and genetic characteristics of G. duodenalis in pet chipmunks in China is limited. The present study therefore aimed to investigate the prevalence and genotypes of G. duodenalis in pet chipmunks in Sichuan province, southwestern China, as well as to assess zoonotic potential of revealed assemblages.
A total of 279 fecal samples were collected from pet chipmunks in seven pet shops and one breeding facility in Sichuan province, southwestern China. The prevalence of G. duodenalis was 8.6% (24/279), as determined by nested PCR detection of the beta giardin (bg) gene. Giardia duodenalis assemblages and subtypes were determined using multilocus genotyping of the bg, triosephosphate isomerase (tpi), and glutamate dehydrogenase (gdh) loci. Two assemblages were identified: potentially zoonotic assemblage A (54.2%, 13/24) and rodent-specific assemblage G (45.8%, 11/24). A total of 24, 17 and 17 sequences of the bg, gdh and tpi loci, respectively, were successfully obtained, which formed four, four and three subtypes, respectively. Moreover, four assemblage A (MLGs A1-A4) and three assemblage G (MLGs G1-G3) multilocus genotypes were identified.
To our knowledge, this is the first study that investigated G. duodenalis in pet chipmunks in China. Detection of assemblage A in pet chipmunks and in previous studies in humans suggests a possible role of chipmunks as a reservoir for human giardiasis in Sichuan Province, China.
十二指肠贾第鞭毛虫是一种鞭毛寄生虫，可导致人类和其他动物腹泻。尽管在世界各地的伴侣动物中都发现了 G. 十二指肠，但关于中国宠物花栗鼠中 G. 十二指肠的流行率和遗传特征的信息有限。因此，本研究旨在调查中国西南部四川省宠物花栗鼠中 G. 十二指肠的流行率和基因型，并评估所揭示的聚集物的人畜共患潜力。
从四川省 7 家宠物店和 1 家繁殖场共采集了 279 份宠物花栗鼠粪便样本。通过巢式 PCR 检测β贾第虫（bg）基因，确定 G. 十二指肠的流行率为 8.6%（24/279）。通过 bg、三磷酸甘油醛异构酶（tpi）和谷氨酸脱氢酶（gdh）基因的多位点基因分型确定了贾第虫的聚集和亚型。确定了两种聚集：潜在的人畜共患病聚集 A（54.2%，13/24）和啮齿动物特异性聚集 G（45.8%，11/24）。成功获得了 bg、gdh 和 tpi 基因座的 24、17 和 17 个序列，分别形成了 4、4 和 3 个亚型。此外，还鉴定了 4 个聚集 A（MLGs A1-A4）和 3 个聚集 G（MLGs G1-G3）多基因座基因型。
据我们所知，这是首次在中国宠物花栗鼠中调查 G. 十二指肠。在宠物花栗鼠和之前在人类中进行的研究中检测到聚集物 A 表明，花栗鼠可能在中国四川省成为人类贾第虫病的储主。
