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中国警犬和农场犬体内十二指肠贾第虫分离株的分子特征。

Molecular characterization of Giardia duodenalis isolates from police and farm dogs in China.

机构信息

College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.

出版信息

Exp Parasitol. 2013 Oct;135(2):223-6. doi: 10.1016/j.exppara.2013.07.009. Epub 2013 Jul 25.

Abstract

To assess the potential zoonotic transmission of giardiasis from dogs in China, a total of 205 fecal specimens from dogs were screened for Giardia duodenalis using PCR and sequence analysis of the triosephosphate isomerase gene. The prevalence of G. duodenalis in dogs was 13.2% (27/205). The potentially zoonotic assemblage A and the dog-specific assemblage C was identified in 25 (12.2%) and two (1.0%) dogs, respectively. All assemblage A isolates belonged to sub-assemblage AI, genotype AI-1. Likewise, one subtype was found in assemblage C. The high occurrence of potentially zoonotic G. duodenalis subtype AI-1 in dogs that are in close contact with humans is of public health concern.

摘要

为了评估中国犬源贾第虫病的潜在人畜共患病传播风险,采用 PCR 和三磷酸甘油醛异构酶基因序列分析的方法对 205 份犬粪便样本进行了贾第虫检测。结果显示,犬源贾第虫的感染率为 13.2%(27/205)。共鉴定出 25 份(12.2%)和 2 份(1.0%)犬源潜在人畜共患病源聚集 A 和犬特异性聚集 C。所有聚集 A 分离株均属于亚聚集 AⅠ,基因型 AI-1。同样,在聚集 C 中也发现了一个亚型。与人密切接触的犬中高频率存在潜在人畜共患病源的贾第虫亚型 AI-1,这引起了公共卫生关注。

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