Department of Aquatic Life Medicine, Kunsan National University, Kunsan, Chunbuk, Republic of Korea.
Fish Shellfish Immunol. 2012 Jun;32(6):1016-21. doi: 10.1016/j.fsi.2012.02.023. Epub 2012 Mar 3.
The purpose of the present study was to evaluate the effect of dietary mistletoe extracts on non-specific immune response and disease resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila infection. Tilapia fingerlings were fed with a diet containing 0 mg as a control, 10 mg, 50 mg, and 200 mg mistletoe powder kg(-1) dry diet for 80 days. The immunological parameters, respiratory burst activity, lysozyme activity, alternative complement haemolysis activity (ACH(50)), and phagocytic activity of fish were investigated following 20, 40 and 80 days of feeding. Fish were challenged with A. hydrophila on 80 days after feeding and mortalities were checked over 10 days post-infection. The results show that fish fed with mistletoe extract exhibited an increase in activity in all immunological parameters (P < 0.05) compared to the control group depending on feeding periods and doses of mistletoe. Following challenge with A. hydrophila, 42% less survivability was observed in the control group than in other experimental diet groups. The highest survival rate (83%) was shown in the group fed with a 50 mg mistletoe kg(-1) diet. The results suggest that mistletoe enables tilapia to promote immunity and be more resistant to A. hydrophila infection.
本研究旨在评估饮食槲寄生提取物对尼罗罗非鱼（Oreochromis niloticus）非特异性免疫反应和对嗜水气单胞菌感染的抗病能力的影响。将罗非鱼鱼苗用含有 0 mg（作为对照）、10 mg、50 mg 和 200 mg 槲寄生粉 kg(-1)干饲料的饮食喂养 80 天。在喂养 20、40 和 80 天后，研究了鱼类的免疫参数、呼吸爆发活性、溶菌酶活性、替代补体溶血活性（ACH(50)）和吞噬活性。在喂养 80 天后用嗜水气单胞菌对鱼进行攻毒，感染后 10 天内检查死亡率。结果表明，与对照组相比，根据喂养期和槲寄生剂量，摄食槲寄生提取物的鱼的所有免疫参数的活性均增加（P < 0.05）。在受到嗜水气单胞菌攻击后，对照组的存活率比其他实验组低 42%。在摄食 50 mg/kg 槲寄生的组中，存活率最高（83%）。结果表明，槲寄生使罗非鱼能够促进免疫并对嗜水气单胞菌感染更具抵抗力。
