Aly Salah Mesalhy, Abdel-Galil Ahmed Yousef, Abdel-Aziz Ghareeb Ahlam, Mohamed Moahmed Fathi
Department of Fish Health, WorldFish Center, Regional Research Center for Africa and West Asia, Abbassa, Sharkia, Egypt.
Fish Shellfish Immunol. 2008 Jul;25(1-2):128-36. doi: 10.1016/j.fsi.2008.03.013. Epub 2008 Mar 28.
The probiotic activity of two bacteria (Bacillus subtilis and Lactobacillus acidophilus) was evaluated by its effect on the immune response of Nile tilapia (Oreochromis niloticus), beside its protective effect against challenge infections. Furthermore, their in-vitro inhibitory activity was evaluated. The in-vitro antimicrobial assay showed that Bacillus subtilis and Lactobacillus acidophilus inhibited the growth of A. hydrophila. The B. subtilis inhibited the development of P. fluorescens while L. acidophilus inhibited the growth of Strept. iniae. The B. subtilis and L. acidophilus proved harmless when injected in the O. niloticus. The feed, containing a mixture of B. subtilis and L. acidophilus or B. subtilis alone, showed significantly greater numbers of viable cells than feed containing L. acidophilus only after 1, 2, 3 and 4 weeks of storage at 4 degrees C and 25 degrees C. The survival rate and the body-weight gain were significantly increased in the fish given B. subtilis and L. acidophilus for one and two months after application. The hematocrit values showed a significant increase in the group that received the mixture of B. subtilis and L. acidophilus compared with the control group. The nitroblue tetrazolium (NBT) assay, neutrophil adherence and lysozyme activity, showed a significant increase in all the probiotic-treated groups after 1 and 2 months of feeding, when compared with the untreated control group. The serum bactericidal activity was high in the group that was given a mixture of the two bacteria. The relative level of protection (RLP) was significantly higher against A. hydrophila, in the bacterial mixture treated group and against P. fluorescens in the L. acidophilus treated group, after one month of the feeding trial. A significantly higher RLP, against A. hydrophila or P. fluorescens, was noticed after 2 months of the feeding trial in the group given a mixture of the two bacteria, and against Strept. iniae in the group fed a diet containing L. acidophilus.
评估了两种细菌（枯草芽孢杆菌和嗜酸乳杆菌）的益生菌活性，包括其对尼罗罗非鱼（尼罗口孵非鲫）免疫反应的影响以及对攻毒感染的保护作用。此外，还评估了它们的体外抑制活性。体外抗菌试验表明，枯草芽孢杆菌和嗜酸乳杆菌抑制嗜水气单胞菌的生长。枯草芽孢杆菌抑制荧光假单胞菌的生长，而嗜酸乳杆菌抑制海豚链球菌的生长。将枯草芽孢杆菌和嗜酸乳杆菌注射到尼罗口孵非鲫体内后证明是无害的。在4℃和25℃下储存1、2、3和4周后，含有枯草芽孢杆菌和嗜酸乳杆菌混合物或仅含有枯草芽孢杆菌的饲料显示出的活细胞数量明显多于仅含有嗜酸乳杆菌的饲料。在投喂枯草芽孢杆菌和嗜酸乳杆菌1个月和2个月后，鱼的存活率和体重增加显著提高。与对照组相比，接受枯草芽孢杆菌和嗜酸乳杆菌混合物的组血细胞比容值显著增加。在投喂1个月和2个月后，与未处理的对照组相比，所有益生菌处理组的硝基蓝四唑（NBT）试验、中性粒细胞黏附及溶菌酶活性均显著增加。给予两种细菌混合物的组血清杀菌活性较高。在投喂试验1个月后，细菌混合物处理组对嗜水气单胞菌的相对保护水平（RLP）显著较高，嗜酸乳杆菌处理组对荧光假单胞菌的RLP显著较高。在投喂试验2个月后，给予两种细菌混合物的组对嗜水气单胞菌或荧光假单胞菌的RLP显著较高，而投喂含有嗜酸乳杆菌饲料的组对海豚链球菌的RLP显著较高。
