Department of Clinical Pathology, Collage of Veterinary Medicine, Mansoura University, 1 Ahmed Mahar, Street, Mansoura, Egypt.
Fish Shellfish Immunol. 2010 May-Jun;28(5-6):802-8. doi: 10.1016/j.fsi.2010.01.017. Epub 2010 Feb 6.
Six hundred and forty Nile tilapia (Oreochromis niloticus) weighing 80-100g were randomly allocated into eight equal groups (80 each). The first group acts as control. Groups S, B and L were fed on a ration supplemented with Saccharomyces cerevisiae, beta-glucans and laminaran, respectively for 21 days. Groups M, MS, MB and ML were subjected throughout the experiment to sublethal concentration of mercuric chloride (0.05 ppm). Gps. MS, MB and ML were fed on a ration containing S. cerevisiae, beta-glucan and laminaran respectively for 21 days. Fish were challenged with Aeromonas hydrophila (0.4 x 10(7) cells mL(-1)) via intra-peritoneal injection and the mortality rate was recorded up to 10 day post-challenge. The non-specific defense mechanisms, cellular and humoral immunity, beside the total and differential leukocytic count were determined. Lymphocyte transformation index, phagocytic activity percent, phagocytic index, total lymphocyte count, serum bactericidal activity and nitric oxide as well as the survival rate were insignificantly changed after 21 day in gps. MS&ML, when compared with mercuric chloride immune depressed group M. These parameters as well as the neutrophil adhesion, serum nitric oxide and survival rate were significantly increased in gp. MB when compared with gp. M. Meanwhile the cellular and humoral immunity beside the survival rate were significantly increased in groups S, B, L when compared with control group. It could be concluded that the whole yeast S. cerevisiae, beta-glucan and laminaran can be used as immunostimulants for the farmed Nile tilapia. The beta-glucans could be used in farmed Nile tilapia, under immune depressive stressful condition to increase their resistance to diseases.
六百四十尾尼罗罗非鱼（Oreochromis niloticus）重 80-100g 被随机分配到八个相等的组（每组 80 尾）。第一组作为对照。S、B 和 L 组分别投喂添加酿酒酵母、β-葡聚糖和昆布多糖的饲料 21 天。M、MS、MB 和 ML 组在整个实验过程中受到亚致死浓度的氯化汞（0.05ppm）的影响。MS、MB 和 ML 组在投喂含酿酒酵母、β-葡聚糖和昆布多糖的饲料 21 天后，通过腹腔注射受到嗜水气单胞菌（0.4×10（7）细胞 mL（-1））的挑战，记录死亡率直至攻毒后 10 天。测定非特异性防御机制、细胞和体液免疫以及总白细胞和分类白细胞计数。淋巴细胞转化指数、吞噬活性百分比、吞噬指数、总淋巴细胞计数、血清杀菌活性和一氧化氮以及存活率在 MS 和 ML 组与免疫抑制组 M 相比，在 21 天后没有显著变化。与 M 组相比，MB 组的中性粒细胞黏附、血清一氧化氮和存活率显著增加。同时，与对照组相比，S、B 和 L 组的细胞和体液免疫以及存活率均显著增加。可以得出结论，整个酵母酿酒酵母、β-葡聚糖和昆布多糖可用作养殖尼罗罗非鱼的免疫刺激剂。在免疫抑制应激条件下，β-葡聚糖可用于养殖尼罗罗非鱼，以提高其对疾病的抵抗力。
