Institute of Forensic Medicine, Stiftsplatz 12, D-53111 Bonn, Germany.
Forensic Sci Int. 2012 Sep 10;221(1-3):98-101. doi: 10.1016/j.forsciint.2012.04.009. Epub 2012 May 1.
There is no toxicological analysis of gamma-hydroxybutyrate (GHB) applied routinely in cases of driving under influence (DUI); therefore the extent of consumption of this drug might be underestimated. Its consumption is described as occurring often concurrently with amphetamine or ecstasy. This study examines 196 serum samples which were collected by police during road side testing for GHB. The samples subject to this study have already been found to be positive for amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA) and/or 3,4-methylenedioxyethamphetamine (MDEA). Analysis has been performed by LC/MS/MS in the multiple reaction monitoring (MRM) mode. Due to its polarity, chromatographic separation of GHB was achieved by a HILIC column. To differentiate endogenous and exogenous levels of GHB, a cut-off concentration of 4μg/ml was applied. Of the 196 samples, two have been found to be positive for GHB. Of these samples, one sample was also positive for amphetamine and one for MDMA. Whilst other amphetamine derivates were not detected in these samples, both samples were found to be positive for cannabinoids. These results suggest that co-consumption of GHB with amphetamine or ecstasy is relatively low (1%) for the collective of this study.
目前在涉及酒后驾车（DUI）的案例中，尚未对γ-羟基丁酸（GHB）进行常规的毒理学分析；因此，该药物的实际使用量可能被低估了。据报道，GHB 的使用常常与安非他命或摇头丸同时发生。本研究检查了 196 份血清样本，这些样本是警方在路边测试 GHB 时收集的。本研究中的样本已经被发现呈安非他命、甲基苯丙胺、3,4-亚甲二氧基苯丙胺（MDA）、3,4-亚甲二氧基甲基苯丙胺（MDMA）和/或 3,4-亚甲二氧基乙基苯丙胺（MDEA）阳性。分析是通过 LC/MS/MS 在多重反应监测（MRM）模式下进行的。由于其极性，GHB 的色谱分离通过亲水作用色谱柱实现。为了区分内源性和外源性 GHB 水平，应用了 4μg/ml 的截止浓度。在 196 个样本中，有两个样本呈 GHB 阳性。其中一个样本还呈安非他命阳性，另一个样本呈 MDMA 阳性。虽然在这些样本中未检测到其他安非他命衍生物，但两个样本均呈大麻素阳性。这些结果表明，在本研究的样本中，GHB 与安非他命或摇头丸的共同使用相对较低（1%）。
