P-糖蛋白在与肌上皮细胞相关的犬乳腺肿瘤中的表达。

P-glycoprotein expression in canine mammary gland tumours related with myoepithelial cells.

机构信息

Department of Veterinary Pathology, Small Animal Tumour Diagnostic Centre, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.

出版信息

Res Vet Sci. 2012 Dec;93(3):1346-52. doi: 10.1016/j.rvsc.2012.04.004. Epub 2012 May 1.

DOI:10.1016/j.rvsc.2012.04.004

PMID:22554937

Abstract

P-glycoprotein is influential in chemotherapy-resistance in numerous cancers and has been widely studied in human breast cancer research, but is less studied in canine mammary gland tumour (MGT). The study was to evaluate P-glycoprotein expression and its localisations related with prognostic factors with monoclonal antibody C219, by immunohistochemistry (IHC) of 68 cases of canine malignant (n=54) and benign (n=14) MGT. Additional immunofluorescence (IF) and reverse transcriptase-polymerase chain reaction (RT-PCR) were also performed. There was a novel finding that P-glycoprotein expression with C219 localised at two different cell types: epithelial and myoepithelial cells. Myoepithelial localised tumours were 5 benign (35.5%) and 21 malignant (63.6%), while epithelial localised tumours were 12 cases, all malignant (36.5%). Unlike conventional belief, semi-quantitative evaluation of IHC intensity scores of C219 expression in malignant MGT was related with favourable histopathological parameters.

摘要

P-糖蛋白在许多癌症的化疗耐药中具有重要作用，并在人类乳腺癌研究中得到了广泛研究，但在犬乳腺肿瘤（MGT）中的研究较少。本研究旨在通过免疫组织化学（IHC）检测 68 例犬恶性（n=54）和良性（n=14）MGT 中单克隆抗体 C219 的 P-糖蛋白表达及其与预后因素的定位，评估 P-糖蛋白的表达。还进行了额外的免疫荧光（IF）和逆转录-聚合酶链反应（RT-PCR）。有一个新的发现，C219 定位的 P-糖蛋白表达位于两种不同的细胞类型：上皮细胞和肌上皮细胞。肌上皮细胞定位的肿瘤有 5 例良性（35.5%）和 21 例恶性（63.6%），而上皮细胞定位的肿瘤有 12 例，均为恶性（36.5%）。与传统观点不同的是，恶性 MGT 中 C219 表达的 IHC 强度评分的半定量评估与良好的组织病理学参数有关。