School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
Chembiochem. 2012 May 29;13(8):1121-4. doi: 10.1002/cbic.201200167. Epub 2012 May 3.
Nonenzymatic DNA ligation chemistries containing a reversible step allow thermodynamic control of product formation, but they are not necessarily compatible with polymerase enzymes. We report a ligation system that uses commercially available reagents, includes a reversible step, and results in a linkage that can function as a template for PCR amplification with accurate sequence transfer.
包含可逆步骤的非酶 DNA 连接化学可通过热力学控制产物形成，但不一定与聚合酶酶兼容。我们报告了一种使用市售试剂、包含可逆步骤的连接系统，其产生的连接物可以作为 PCR 扩增的模板，并且具有准确的序列转移。
