评估巴西感染犬利什曼原虫分离株的遗传多样性。
An assessment of the genetic diversity of Leishmania infantum isolates from infected dogs in Brazil.
机构信息
Laboratório de Patologia e Biointervenção do CPqGM, FIOCRUZ, Bahia, Brazil.
出版信息
Am J Trop Med Hyg. 2012 May;86(5):799-806. doi: 10.4269/ajtmh.2012.11-0300.
Abstract
Correlations between the genetic diversity of Leishmania infantum (syn. L. chagasi) isolates and their respective geographic origins support the theoretic assumption that visceral leishmaniasis probably originated in the Old World. Because dogs are widely considered to be the main reservoir of this disease, the present study aimed to investigate the degree of genetic divergence among 44 leishmanial canine isolates from two Brazilian cities, Jequié and Campo Grande, located approximately 2,028 km from each other. We hypothesized that a low degree of genetic divergence would be observed among these isolates. In fact, statistical analyses found no significant differences between the isolates using both random amplified polymorphic DNA and multilocus microsatellite typing genotyping techniques with three and seven markers, respectively. These findings provide support for the recent introduction of L. infantum into the New World.
摘要
利什曼原虫（即恰加斯原虫）分离株的遗传多样性与其各自的地理起源之间的相关性支持这样一种理论假设，即内脏利什曼病可能起源于旧世界。由于狗被广泛认为是这种疾病的主要宿主，本研究旨在调查来自巴西两个城市——若基埃和坎波格兰德的 44 株犬利什曼原虫分离株之间的遗传分化程度。我们假设这些分离株之间的遗传分化程度较低。事实上，使用随机扩增多态性 DNA 和多位点微卫星分型两种技术，分别使用 3 个和 7 个标记进行的统计分析发现，这些分离株之间没有显著差异。这些发现为利什曼原虫最近传入新世界提供了支持。
相似文献
1
An assessment of the genetic diversity of Leishmania infantum isolates from infected dogs in Brazil.评估巴西感染犬利什曼原虫分离株的遗传多样性。
Am J Trop Med Hyg. 2012 May;86(5):799-806. doi: 10.4269/ajtmh.2012.11-0300.
2
Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employing multilocus microsatellite typing directly in dog infected tissues.巴西圣保罗州利什曼原虫基因型的空间分布和种群遗传学研究，采用多基因微卫星直接在感染组织的犬中进行基因分型。
Infect Genet Evol. 2013 Aug;18:48-59. doi: 10.1016/j.meegid.2013.04.031. Epub 2013 May 9.
3
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil.巴西米纳斯吉拉斯州贝洛奥里藏特大都市区（BHMA）犬类和人类样本中婴儿利什曼原虫（利什曼原虫）分离株的遗传同质性。
Parasit Vectors. 2015 Apr 15;8:226. doi: 10.1186/s13071-015-0837-y.
4
First insights into the genetic diversity and origin of Leishmania infantum in Mont Rolland (Thiès region, Senegal).首次深入了解塞内加尔济金绍尔地区龙滚市利什曼原虫的遗传多样性和起源。
Microbes Infect. 2016 Jun;18(6):412-420. doi: 10.1016/j.micinf.2016.02.003. Epub 2016 Mar 3.
5
Multilocus microsatellite typing (MLMT) reveals host-related population structure in Leishmania infantum from northeastern Italy.多位点微卫星分型（MLMT）揭示了意大利东北部利什曼原虫的宿主相关种群结构。
PLoS Negl Trop Dis. 2018 Jul 5;12(7):e0006595. doi: 10.1371/journal.pntd.0006595. eCollection 2018 Jul.
6
The genetic structure of Leishmania infantum populations in Brazil and its possible association with the transmission cycle of visceral leishmaniasis.巴西利什曼原虫种群的遗传结构及其与内脏利什曼病传播周期的可能关联。
PLoS One. 2012;7(5):e36242. doi: 10.1371/journal.pone.0036242. Epub 2012 May 11.
7
KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.通过对新世界和旧世界分离的利什曼原虫（Leishmania） infantum 的 LSSP-PCR 分析获得的 KDNA 遗传特征。
PLoS One. 2012;7(8):e43363. doi: 10.1371/journal.pone.0043363. Epub 2012 Aug 17.
8
Genetic Diversity and Population Structure of Leishmania infantum from Southeastern France: Evaluation Using Multi-Locus Microsatellite Typing.法国东南部婴儿利什曼原虫的遗传多样性和种群结构：使用多位点微卫星分型进行评估
PLoS Negl Trop Dis. 2016 Jan 25;10(1):e0004303. doi: 10.1371/journal.pntd.0004303. eCollection 2016 Jan.
9
Genetic diversity evaluation on Portuguese Leishmania infantum strains by multilocus microsatellite typing.通过多位点微卫星分型对葡萄牙婴儿利什曼原虫菌株进行遗传多样性评估。
Infect Genet Evol. 2014 Aug;26:20-31. doi: 10.1016/j.meegid.2014.04.023. Epub 2014 May 9.
10
Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil.对巴西东北部人类和犬利什曼原虫分离株全基因组序列的比较分析。
Int J Parasitol. 2017 Sep;47(10-11):655-665. doi: 10.1016/j.ijpara.2017.04.004. Epub 2017 Jun 10.
引用本文的文献
1
Genetic variability of Leishmania (Leishmania) infantum causing human visceral leishmaniasis in the Southeastern Brazil.巴西东南部导致人类内脏利什曼病的利什曼原虫（利什曼原虫）的遗传变异性。
Rev Inst Med Trop Sao Paulo. 2023 Oct 20;65:e55. doi: 10.1590/S1678-9946202365055. eCollection 2023.
2
Genetic diversity of Leishmania donovani that causes cutaneous leishmaniasis in Sri Lanka: a cross sectional study with regional comparisons.斯里兰卡引起皮肤利什曼病的杜氏利什曼原虫的遗传多样性：一项区域比较的横断面研究
BMC Infect Dis. 2017 Dec 22;17(1):791. doi: 10.1186/s12879-017-2883-x.
3
Leishmania infantum Genetic Diversity and Lutzomyia longipalpis Mitochondrial Haplotypes in Brazil.巴西婴儿利什曼原虫的遗传多样性及长须罗蛉的线粒体单倍型
Biomed Res Int. 2016;2016:9249217. doi: 10.1155/2016/9249217. Epub 2016 Mar 29.
4
Leishmania donovani P23 protects parasites against HSP90 inhibitor-mediated growth arrest.杜氏利什曼原虫P23可保护寄生虫免受热休克蛋白90（HSP90）抑制剂介导的生长停滞影响。
Cell Stress Chaperones. 2015 Jul;20(4):673-85. doi: 10.1007/s12192-015-0595-y. Epub 2015 May 7.
5
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil.巴西米纳斯吉拉斯州贝洛奥里藏特大都市区（BHMA）犬类和人类样本中婴儿利什曼原虫（利什曼原虫）分离株的遗传同质性。
Parasit Vectors. 2015 Apr 15;8:226. doi: 10.1186/s13071-015-0837-y.
6
Evaluating the accuracy of molecular diagnostic testing for canine visceral leishmaniasis using latent class analysis.使用潜在类别分析评估犬内脏利什曼病分子诊断检测的准确性。
PLoS One. 2014 Jul 30;9(7):e103635. doi: 10.1371/journal.pone.0103635. eCollection 2014.
本文引用的文献
1
Genetic diversity of Leishmania infantum field populations from Brazil.巴西利什曼原虫野外种群的遗传多样性。
Mem Inst Oswaldo Cruz. 2012 Feb;107(1):39-47. doi: 10.1590/s0074-02762012000100006.
2
Comparative microsatellite typing of new world leishmania infantum reveals low heterogeneity among populations and its recent old world origin.新世界利什曼原虫的比较微卫星分型揭示了种群间的低异质性及其最近的旧世界起源。
PLoS Negl Trop Dis. 2011 Jun;5(6):e1155. doi: 10.1371/journal.pntd.0001155. Epub 2011 Jun 7.
3
Guns, germs and dogs: On the origin of Leishmania chagasi.枪炮、病菌与钢铁：在查加斯原虫起源上的探讨
Infect Genet Evol. 2011 Jul;11(5):1091-5. doi: 10.1016/j.meegid.2011.04.004. Epub 2011 Apr 12.
4
Heterogeneity of Leishmania infantum chagasi kinetoplast DNA in Teresina (Brazil).巴西特蕾西纳地区查加斯利什曼原虫动基体 DNA 的异质性。
Am J Trop Med Hyg. 2010 May;82(5):819-21. doi: 10.4269/ajtmh.2010.09-0600.
5
Development and evaluation of different PCR-based typing methods for discrimination of Leishmania donovani isolates from Nepal.建立并评估不同的基于 PCR 的分子分型方法以区分尼泊尔的利什曼原虫分离株。
Parasitology. 2010 May;137(6):947-57. doi: 10.1017/S0031182009991752. Epub 2010 Jan 29.
6
Current Brazilian law on animal experimentation.巴西现行的动物实验相关法律。
Science. 2009 Jun 26;324(5935):1643-4. doi: 10.1126/science.324_1643.
7
Demonstration of genetic exchange during cyclical development of Leishmania in the sand fly vector.利什曼原虫在白蛉媒介中周期性发育过程中的基因交换证明。
Science. 2009 Apr 10;324(5924):265-8. doi: 10.1126/science.1169464.
8
Arlequin (version 3.0): an integrated software package for population genetics data analysis.Arlequin（版本 3.0）：一个用于群体遗传学数据分析的集成软件包。
Evol Bioinform Online. 2007 Feb 23;1:47-50.
9
Differentiation and gene flow among European populations of Leishmania infantum MON-1.欧洲利什曼原虫 MON-1 种群的分化和基因流动。
PLoS Negl Trop Dis. 2008 Jul 9;2(7):e261. doi: 10.1371/journal.pntd.0000261.
10
Canine leishmaniosis - new concepts and insights on an expanding zoonosis: part one.犬利什曼病——关于一种不断蔓延的人畜共患病的新概念与见解：第一部分
Trends Parasitol. 2008 Jul;24(7):324-30. doi: 10.1016/j.pt.2008.04.001. Epub 2008 May 29.
Zotero 插件