Laboratório de Patologia e Biointervenção do CPqGM, FIOCRUZ, Bahia, Brazil.
Am J Trop Med Hyg. 2012 May;86(5):799-806. doi: 10.4269/ajtmh.2012.11-0300.
Correlations between the genetic diversity of Leishmania infantum (syn. L. chagasi) isolates and their respective geographic origins support the theoretic assumption that visceral leishmaniasis probably originated in the Old World. Because dogs are widely considered to be the main reservoir of this disease, the present study aimed to investigate the degree of genetic divergence among 44 leishmanial canine isolates from two Brazilian cities, Jequié and Campo Grande, located approximately 2,028 km from each other. We hypothesized that a low degree of genetic divergence would be observed among these isolates. In fact, statistical analyses found no significant differences between the isolates using both random amplified polymorphic DNA and multilocus microsatellite typing genotyping techniques with three and seven markers, respectively. These findings provide support for the recent introduction of L. infantum into the New World.
利什曼原虫（即恰加斯原虫）分离株的遗传多样性与其各自的地理起源之间的相关性支持这样一种理论假设，即内脏利什曼病可能起源于旧世界。由于狗被广泛认为是这种疾病的主要宿主，本研究旨在调查来自巴西两个城市——若基埃和坎波格兰德的 44 株犬利什曼原虫分离株之间的遗传分化程度。我们假设这些分离株之间的遗传分化程度较低。事实上，使用随机扩增多态性 DNA 和多位点微卫星分型两种技术，分别使用 3 个和 7 个标记进行的统计分析发现，这些分离株之间没有显著差异。这些发现为利什曼原虫最近传入新世界提供了支持。
