McNeilly Jane D, Mason Avril, Khanna Sheila, Galloway Peter J, Ahmed S Faisal
Developmental Endocrinology Research Group, Royal Hospital for Sick Children, Glasgow, UK.
Int J Pediatr Endocrinol. 2012 May 4;2012(1):10. doi: 10.1186/1687-9856-2012-10.
Non-invasive screening investigations are rarely used for assessing the activation and progression of the hypothalamic-pituitary gonadal axis through puberty. This study aimed to establish a normal range for urinary gonadotrophins in children progressing through puberty.
Urine samples were collected from 161 healthy school children (76 boys, 85 girls) aged 4-19 yrs. Height and weight were converted to standard deviation score. Pubertal status, classified by Tanner staging, was determined by self-assessment. Urinary gonadotrophins were measured by chemiluminescent microparticle immunoassay. Results were grouped according to pubertal status (pre-pubertal or pubertal).
Of the 161 children, 50 were pre-pubertal (28 boys; 22 girls) and 111 were pubertal (48 boys; 63 girls). Overall, urinary gonadotrophins concentrations increased with pubertal maturation. All pre-pubertal children had a low urinary LH:Creatinine ratio. LH:Creatinine ratios were significantly higher in pubertal compared to pre-pubertal boys (p<0.001). In girls, FSH:Creatinine ratios were significantly higher in the pubertal group (p = 0.006). However, LH:FSH ratios were a more consistent discriminant between pre-pubertal and pubertal states in both sexes (Boys 0.45 pubertal vs 0.1 pre-pubertal; girls 0.23 pubertal vs 0.06 pre-pubertal).
Urinary gonadotrophins analyses could be used as non-invasive integrated measurement of pubertal status which reflects clinical/physical status.
非侵入性筛查研究很少用于评估下丘脑 - 垂体 - 性腺轴在青春期的激活和进展情况。本研究旨在确定青春期发育儿童尿促性腺激素的正常范围。
收集了161名4至19岁健康在校儿童（76名男孩，85名女孩）的尿液样本。身高和体重转换为标准差评分。通过自我评估确定按坦纳分期分类的青春期状态。采用化学发光微粒子免疫分析法测量尿促性腺激素。结果根据青春期状态（青春期前或青春期）分组。
161名儿童中，50名处于青春期前（28名男孩；22名女孩），111名处于青春期（48名男孩；63名女孩）。总体而言，尿促性腺激素浓度随着青春期成熟而增加。所有青春期前儿童的尿促黄体生成素：肌酐比值较低。与青春期前男孩相比，青春期男孩的促黄体生成素：肌酐比值显著更高（p<0.001）。在女孩中，青春期组的促卵泡生成素：肌酐比值显著更高（p = 0.006）。然而，促黄体生成素：促卵泡生成素比值在区分两性青春期前和青春期状态方面更为一致（男孩青春期为0.45，青春期前为0.1；女孩青春期为0.23，青春期前为0.06）。
尿促性腺激素分析可作为反映临床/身体状况的青春期状态的非侵入性综合测量方法。
