University of Arizona, College of Optical Sciences, 1630 East University Boulevard, Tuscon, Arizona 85721, USA.
J Biomed Opt. 2012 Apr;17(4):046004. doi: 10.1117/1.JBO.17.4.046004.
Ratiometric fluorescence-imaging technique is commonly used to measure extracellular pH in tumors and surrounding tissue within a dorsal skin-fold window chamber. Using a pH-sensitive fluorophore such as carboxy SNARF-1 one can measure pH distributions with high precision. However, it is often observed that the measured pH is lower than expected, with a bias that varies from one image to another. A comprehensive analysis of possible error sources is presented. These error sources include photon noise, estimator bias, instrument errors, temperature, and calibration errors from biological factors.
比率荧光成像技术常用于测量背皮窗室肿瘤及周围组织的细胞外 pH 值。通过使用羧基 SNARF-1 等 pH 敏感荧光探针，可以高精度测量 pH 值分布。然而，我们常常发现测量的 pH 值比预期值低，并且这种偏差在不同图像间存在差异。本文全面分析了可能的误差来源，包括光子噪声、估计偏差、仪器误差、温度以及生物因素引起的校准误差。
