Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Alice, South Africa.
Arch Med Res. 2012 Apr;43(3):197-204. doi: 10.1016/j.arcmed.2012.04.006. Epub 2012 May 3.
Alternative therapy for Helicobacter pylori eradication from natural products is gaining much attention. This study sought to isolate and characterize the fraction responsible for the antibacterial activity in Goldcrest (GC) n-hexane extract.
Thin-layer chromatography (TLC) of the extract was carried out on Silica gel plates to determine the presence of chemical compounds, which were separated and partially purified by column chromatography. The obtained fractions GCCL, GCF2, GCF3 and GCF4 were tested for anti-H. pylori activity using the broth microdilution method. Volatile compounds in the active fractions were identified by gas chromatography-mass spectrometry (GC-MS) analysis. MINITAB was used for statistical analysis at 95% confidence interval.
The best antibacterial activity was exhibited by GCF3 (5 mg/mL), which was composed of many compounds with known antimicrobial and antioxidant properties. A total of 16 volatile compounds were identified from fractions GCF2, GCF3 and GCF4 into the following families; alcohol, ketone, aliphatic acid, benzene compound, hydrocarbon, furan and pyran derivatives.
The demonstration of antibacterial activity by the column fractions of GC n-hexane extract may provide new lead molecules that could serve as selective agents for H. pylori chemotherapy and control.
从天然产物中寻找替代幽门螺杆菌根除的治疗方法正受到越来越多的关注。本研究旨在从金顶杜鹃（GC）正己烷提取物中分离并鉴定具有抗菌活性的部位。
采用薄层色谱（TLC）法对提取物进行分析，以确定是否存在化合物，然后采用柱色谱法对其进行分离和部分纯化。用肉汤微量稀释法检测所得 GC 氯仿提取物（GCCL）、GCF2、GCF3 和 GCF4 对幽门螺杆菌的抑制活性。采用气相色谱-质谱联用（GC-MS）分析法对活性部位的挥发性化合物进行鉴定。采用 MINITAB 在 95%置信区间进行统计分析。
GCF3（5mg/ml）具有最佳的抗菌活性，其包含许多具有已知抗菌和抗氧化特性的化合物。从 GCF2、GCF3 和 GCF4 等分中鉴定出 16 种挥发性化合物，分为醇、酮、脂肪酸、苯化合物、烃类、呋喃和吡喃衍生物。
GC 正己烷提取物柱分馏物的抗菌活性证明，其可能为幽门螺杆菌化学疗法和控制提供新的先导分子。
