Manyi-Loh Christy Echakachi, Clarke Anna Maria, Green Ezekiel, Ndip Roland Ndip
Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, P/Bag X1314, Alice, South Africa.
Pak J Pharm Sci. 2013 Sep;26(5):897-906.
The growing problem of antibiotic resistance by Helicobacter pylori demands the search for novel compounds, especially from natural sources. We evaluated the anti-H. pylori activity of six local honeys at different concentrations as well as their solvent extracts by the Hole Plate diffusion method. The minimum inhibitory concentration (MIC50) of the two most active extracts of each honey was determined by the broth microdilution method; and the time kill assay of the most active extract of each honey determined by viability studies. Data were analyzed by one-way ANOVA test at 95% significance level. All the honey varieties as well as their solvent extracts demonstrated varying levels of antibacterial activity based on different mean zone diameters [16.0mm (crude) to 22.2mm (extract)] and percentage susceptibilities [73.3% (crude) to 93.3% (extract)] of the test isolates. The chloroform extracts of Pure Honey (PH) and Champagne Royal Train (CRT) recorded MIC50 ranges of 0.01-10% and 0.625-10 % (v/v) respectively; that were not significantly different (P > 0.05) from amoxicillin (0.001-1.25mg/mL), the positive control. The most potent bactericidal effect against the test isolates was obtained with 5% v/v (1/2 MIC) concentration of chloroform extract of PH from 42-72 hrs. In conclusion, these honeys and their extracts could be leads for further investigation in the discovery of new natural anti-H. pylori compounds.
幽门螺杆菌抗生素耐药性问题日益严重，这就需要寻找新型化合物，尤其是从天然来源中寻找。我们采用孔板扩散法评估了六种本地蜂蜜在不同浓度下及其溶剂提取物的抗幽门螺杆菌活性。通过肉汤微量稀释法测定每种蜂蜜两种活性最强提取物的最低抑菌浓度（MIC50）；通过活力研究确定每种蜂蜜活性最强提取物的杀菌时间测定。数据采用95%显著性水平的单因素方差分析进行分析。所有蜂蜜品种及其溶剂提取物基于测试菌株不同的平均抑菌圈直径[16.0毫米（粗提物）至22.2毫米（提取物）]和敏感性百分比[73.3%（粗提物）至93.3%（提取物）]表现出不同程度的抗菌活性。纯蜂蜜（PH）和香槟皇家列车蜂蜜（CRT）的氯仿提取物的MIC50范围分别为0.01 - 10%和0.625 - 10%（v/v）；与阳性对照阿莫西林（0.001 - 1.25毫克/毫升）相比无显著差异（P > 0.05）。用5% v/v（1/2 MIC）浓度的PH氯仿提取物在42 - 72小时对测试菌株产生了最有效的杀菌作用。总之，这些蜂蜜及其提取物可能是进一步研究发现新型天然抗幽门螺杆菌化合物的线索。
