Department of Biotechnology & Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, HP, India.
Fitoterapia. 2012 Sep;83(6):1131-7. doi: 10.1016/j.fitote.2012.04.015. Epub 2012 Apr 26.
In light of the economic importance of buckwheat as well as existence of enormous accessions of Fagopyrum species in the Himalayan regions of India, the characterization of tartary buckwheat for rutin content variation vis-à-vis DNA fingerprinting was undertaken so as to identify fingerprint profiles unique to high rutin content accessions. Rutin content analysis in mature seeds of 195 accessions of Fagopyrum tataricum showed a wide range of variation (6 μg/mg to 30 μg/mg D.W.) with most of the accessions (81%) containing 10-16 μg/mg of rutin followed by 14% accessions with significantly higher rutin content (17 μg/mg to 30 μg/mg) and 5% accessions with low rutin content (≤10 μg/mg). AFLP fingerprinting of 18 accessions having high (≥17 μg/mg) and low rutin content (≤10 μg/mg) with 19 EcoRI/MseI primer combinations yielded 136 polymorphic fragments out of total 907. The hierarchical and model-based cluster analyses of AFLP data strongly suggested that the 18 populations of F. tataricum were clustered into two separate groups. The high and low rutin content accessions were clustered into two separate groups based on AFLP fingerprinting. The AFLP fingerprints associated with high rutin content accessions of F. tataricum are expected to be useful for evaluation, conservation and genetic improvement of buckwheat.
鉴于荞麦在经济上的重要性，以及印度喜马拉雅地区存在大量荞麦属植物的资源，本研究对苦荞进行了特征描述，以比较不同品种间的芦丁含量差异，并通过 DNA 指纹图谱进行分析，旨在鉴定高芦丁含量品种的独特指纹图谱。对 195 份苦荞成熟种子的芦丁含量分析表明，其含量变化范围很广（6μg/mg 至 30μg/mgDW），大多数品种（81%）含有 10-16μg/mg 的芦丁，其次是 14%的品种芦丁含量显著较高（17μg/mg 至 30μg/mg），还有 5%的品种芦丁含量较低（≤10μg/mg）。用 19 对 EcoRI/MseI 引物组合对 18 份高芦丁（≥17μg/mg）和低芦丁（≤10μg/mg）含量的苦荞品种进行 AFLP 指纹分析，共得到 136 个多态性片段，占总片段数的 907 个的 15%。基于 AFLP 数据的层次聚类和模型聚类分析强烈表明，18 个苦荞群体可分为两个独立的组。根据 AFLP 指纹图谱，高芦丁和低芦丁含量的苦荞品种可分为两个不同的组。与苦荞高芦丁含量相关的 AFLP 指纹图谱有望对苦荞的评价、保护和遗传改良具有重要意义。
