College of Life Science, Sichuan Agriculture University , Ya'an 625014, Sichuan, People's Republic of China.
J Agric Food Chem. 2012 May 23;60(20):5161-8. doi: 10.1021/jf205192q. Epub 2012 May 14.
Evidence from in vitro and in vivo studies indicates that rutin, the main flavonoid in tartary buckwheat ( Fagopyrum tataricum ), may have high value for medicine and health. This paper reports the finding of a flavonol synthase (FLS) gene, cloned and characterized from F. tataricum and designated FtFLS1, that is involved in rutin biosynthesis. The FtFLS1 gene was expressed in Escherichia coli BL21(DE3), and the recombinant soluble FtFLS1 protein had a relative molecular mass of 40 kDa. The purified recombinant protein showed, with dihydroquercetin as substrate, total and specific activities of 36.55 × 10(-3) IU and 18.94 × 10(-3) IU/mg, respectively, whereas the total and specific activities were 10.19 × 10(-3) IU and 5.28 × 10(-3) IU/mg, respectively, with dihydrokaempferol. RT-PCR revealed that during F. tataricum florescence there was an organ-specific expression pattern by the FtFLS1 gene, with similar trends in flavonoid content. These observations suggest that FtFLS1 in F. tataricum encodes a functional protein, which might play a key role in rutin biosynthesis.
体外和体内研究证据表明，鞑靼荞麦（Fagopyrum tataricum）中的主要类黄酮芦丁可能具有很高的药用和健康价值。本文报道了从鞑靼荞麦中克隆和鉴定的类黄酮合酶（FLS）基因FtFLS1 的发现，该基因参与芦丁生物合成。FtFLS1 基因在大肠杆菌 BL21(DE3)中表达，重组可溶性 FtFLS1 蛋白的相对分子质量为 40 kDa。纯化的重组蛋白以二氢槲皮素为底物，总活性和比活性分别为 36.55×10(-3)IU 和 18.94×10(-3)IU/mg，而以二氢山奈酚为底物时，总活性和比活性分别为 10.19×10(-3)IU 和 5.28×10(-3)IU/mg。RT-PCR 显示，在鞑靼荞麦开花期间，FtFLS1 基因的表达具有器官特异性，类黄酮含量也呈现相似的趋势。这些观察结果表明，鞑靼荞麦中的 FtFLS1 编码一种功能性蛋白，可能在芦丁生物合成中发挥关键作用。
