Guangzhou Fuda Cancer Hospital, No. 2 Tangdexi Road, Tianhe District, Guangzhou 510305, China.
Cryobiology. 2012 Aug;65(1):68-71. doi: 10.1016/j.cryobiol.2012.04.004. Epub 2012 Apr 25.
The purpose of this study was to compare a dual-freeze protocol with a triple-freeze protocol for hepatic cryoablation in a porcine model. Eighteen cryoablations were performed over an exposed operation field in nine normal porcine livers, using dual- (10-5-10-5) and triple-freeze (5-5-5-5-10-5) protocols. Changes in the temperature of the cryoprobes and the diameter of the iceballs were recorded during the ablation, and pathological changes in the cryozones (zones of tissue destruction) were assessed seven days after the procedure. Use of two and three freeze-thaw cycles produced iceballs of different diameters. Seven days after cryosurgery, the triple-freeze protocol was associated with a larger zone of complete necrosis than the dual-freeze protocol, although the two protocols produced cryozones and cryolesions of similar length, and in both cases the cryozones contained five areas of destruction. With the same freezing time (20 min), the triple-freeze protocol may be a more powerful liver ablation method than the dual-freeze protocol.
本研究旨在比较双冻法和三冻法在猪模型中用于肝冷冻消融的效果。在 9 只正常猪的肝脏中，在暴露的手术野上进行了 18 次冷冻消融，使用双冻（10-5-10-5）和三冻（5-5-5-5-10-5）方案。在消融过程中记录冷冻探针的温度和冰球直径的变化，并在手术后 7 天评估冷冻区（组织破坏区）的病理变化。使用两个和三个冻融循环产生了不同直径的冰球。冷冻手术后 7 天，三冻法比双冻法产生的完全坏死区更大，但两种方案产生的冷冻区和冷冻损伤的长度相似，而且在两种情况下，冷冻区都包含五个破坏区。在相同的冷冻时间（20 分钟）下，三冻法可能是一种比双冻法更有效的肝脏消融方法。
