Radetzki F, Wohlrab D, Zeh A, Delank K S, Mendel T, Berger G, Syrowatka F, Mayr O, Bernstein A
Department of Orthopedic Surgery, Martin Luther University Halle-Wittenberg, Saale, Germany.
Biomed Mater Eng. 2011;21(5-6):307-21. doi: 10.3233/BME-2012-0678.
Resorbable ceramics can promote the bony integration of implants. Their rate of degradation should ideally be synchronized with bone regeneration. This study examined the effect of rapidly resorbable calcium phosphate ceramics 602020, GB14, 305020 on adherence, proliferation and morphology of human bone-derived cells (HBDC) in comparison to β-TCP. The in vitro cytotoxicity was determined by the microculture tetrazolium (MTT) assay. HBDC were grown on the materials for 3, 7, 11, 15 and 19 days and counted. Cell morphology, cell attachment, cell spreading and the cytoskeletal organization of HBDC cultivated on the substrates were investigated using laser scanning microscopy and environmental scanning electron microscopy. All substrates supported sufficient cellular growth for 19 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells with strong focal contacts was verified. HBDC showed numerous well defined stress fibres which give proof of well spread and strongly anchored cells. Porous surfaces encouraged the attachment and spreading of HBDC. Further investigations regarding long term biomaterial/cell interactions in vitro and in vivo are required to confirm the utility of the new biomaterials.
可吸收陶瓷可促进种植体的骨结合。理想情况下，其降解速率应与骨再生同步。本研究检测了快速可吸收磷酸钙陶瓷602020、GB14、305020与人骨源细胞（HBDC）的黏附、增殖及形态学的影响，并与β-磷酸三钙进行比较。通过微量培养四氮唑蓝（MTT）法测定体外细胞毒性。将HBDC接种于材料上培养3、7、11、15和19天并计数。使用激光扫描显微镜和环境扫描电子显微镜研究HBDC在底物上的细胞形态、细胞黏附、细胞铺展和细胞骨架组织。所有底物在19天内均支持足够的细胞生长且无细胞毒性。在每种材料上均证实有相同的细胞定植，细胞为相互连通的多边形活细胞，具有强焦点接触。HBDC显示出大量清晰的应力纤维，证明细胞铺展良好且锚定牢固。多孔表面促进了HBDC的黏附和铺展。需要进一步研究新型生物材料在体外和体内的长期生物材料/细胞相互作用，以证实其效用。
