Institute of Basic Medicine, Chinese PLA General Hospital, Beijing, PR China.
Ageing Res Rev. 2013 Jan;12(1):29-38. doi: 10.1016/j.arr.2012.04.002. Epub 2012 Apr 25.
Seeking possible ways to create replacement cells for the faded ones with deficits in functionality or quantity inspires comprehensive needs for cell lineage conversion. To fulfill this promise, reprogramming and microenvironment direction have been used to manipulate abundant cell fates. We briefly describe the evolution and fundamental insights of these two major strategies applied for lineage specification, comment generally on their current limitations, and analyze the orchestral interplay between them. We also present several future directions and discuss the potential clinical uses. Based on the relatively slight safety and technical issues, we conclude that microenvironment-evoked cell lineage conversion, instead of reprogramming, will be the shifting focus in regenerative medicine.
寻求可能的方法来为功能或数量不足的衰退细胞创造替代细胞，这激发了对细胞谱系转换的全面需求。为了实现这一承诺，已经使用重编程和微环境导向来操纵丰富的细胞命运。我们简要描述了这两种主要策略在谱系特化中的应用的演变和基本见解，一般性地评论了它们目前的局限性，并分析了它们之间的协作。我们还提出了几个未来的方向，并讨论了它们的潜在临床应用。基于相对较小的安全性和技术问题，我们得出结论，微环境诱导的细胞谱系转换而不是重编程将成为再生医学的重点转移。
