Department of Burn, 251 Hospital of PLA, Zhangjiakou, Hebei 075000, PR China.
Food Chem Toxicol. 2012 Jul;50(7):2560-4. doi: 10.1016/j.fct.2012.04.033. Epub 2012 Apr 27.
Triptolide(TPT) isolated from one of the Chinese herbs, Tripterygium wilfordii Hook. F. (TWHF), are known to have a variety of immunomodulatory activities. This study was performed to investigate the effect of TPT on the differentiation of splenic DCs and its influence on T cell-mediated immunity regarding to DC subsets CD11c(low)I-a/e(low)CD45RB(+)(CD11c(low) DCs) and CD11c(high)I-a/e(high)CD45RB(-) (CD11c(high) DCs) in male C57BL/6 mice spleens in vitro. The percentage of CD11c(low) DCs was significantly increased after treatment with TPT compared to their counterparts (CD11c(high) DCs). It was found that unlike the gradually decreasing interleukin (IL)-12 secretion of CD11c(high) DCs induced by TPT, CD11c(low) DCs showed a obvious dose-dependent response between the increasing of IL-10 production and TPT stimulation. After treatment with anti-IL-12R or anti-IL-10 monoclonal antibody in CD4(+) T cells+CD11c(high) DCs or CD11c(low) DCs mixed lymphocyte reaction, the induction of these DCs on T cells was inhibited dramatically. These data demonstrated that TPT might induce the differentiation of splenic DCs to CD11c(low) DCs followed by shifting of Th1 to Th2 with enhancement of T lymphocyte immune function in vitro.
雷公藤内酯醇（TPT）是从中国草药雷公藤（TWHF）中分离出来的，已知具有多种免疫调节活性。本研究旨在探讨 TPT 对脾树突状细胞（DCs）分化的影响及其对 T 细胞介导的免疫的影响，具体涉及 DC 亚群 CD11c（low）I-a/e（low）CD45RB（+）（CD11c（low）DCs）和 CD11c（high）I-a/e（high）CD45RB（-）（CD11c（high）DCs）。在体外，雄性 C57BL/6 小鼠脾脏中。与对照组相比，用 TPT 处理后 CD11c（low）DCs 的比例明显增加（CD11c（high）DCs）。结果发现，与 TPT 诱导的 CD11c（high）DCs 中 IL-12 分泌逐渐减少不同，CD11c（low）DCs 在 IL-10 产生增加和 TPT 刺激之间表现出明显的剂量依赖性反应。在 CD4（+）T 细胞+CD11c（high）DCs 或 CD11c（low）DCs 混合淋巴细胞反应中用抗 IL-12R 或抗 IL-10 单克隆抗体处理后，这些 DCs 对 T 细胞的诱导作用明显受到抑制。这些数据表明，TPT 可能诱导脾 DCs 向 CD11c（low）DCs 分化，随后 Th1 向 Th2 转变，增强 T 淋巴细胞免疫功能。
