Laboratory of Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, Rockville, MD 20852, USA.
J Virol Methods. 2012 Aug;183(2):219-23. doi: 10.1016/j.jviromet.2012.04.001. Epub 2012 Apr 25.
Viral adaptation through fixation of spontaneous mutations is an important factor potentially associated with reoccurrence of West Nile virus (WNV) outbreaks in the New World. The emergence of new genetic variants of WNV represents an important public health issue because it may affect the sensitivity of WNV screening and diagnostic assays, as well as the development and efficacy of WNV vaccines and anti-viral drugs. A microarray assay was developed and optimized to enable simple monitoring of WNV genetic variability and rapid detection of any nucleotide mutations within the entire viral genome. The assay was validated using 11 WNV isolates from the 2007 and 2009 U.S. epidemics. The developed microarray system can potentially serve as a high throughput, rapid, and effective approach for the detection of circulating WNV genetic variants.
病毒通过固定自发突变进行适应是一个潜在的重要因素，可能与西尼罗河病毒（WNV）在新世界再次爆发有关。WNV 新的遗传变异体的出现是一个重要的公共卫生问题，因为它可能影响 WNV 筛查和诊断检测的敏感性，以及 WNV 疫苗和抗病毒药物的开发和效果。开发了一种微阵列分析方法，并对其进行了优化，以能够简单地监测 WNV 遗传变异性，并快速检测整个病毒基因组内的任何核苷酸突变。该分析方法使用 2007 年和 2009 年美国流行的 11 个 WNV 分离株进行了验证。开发的微阵列系统可能成为一种高通量、快速和有效的方法，用于检测循环 WNV 遗传变异体。
