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一种基于DNA微阵列的检测两种登革病毒血清型双重感染的检测方法。

A DNA microarray-based assay to detect dual infection with two dengue virus serotypes.

作者信息

Díaz-Badillo Alvaro, Muñoz María de Lourdes, Perez-Ramirez Gerardo, Altuzar Victor, Burgueño Juan, Mendoza-Alvarez Julio G, Martínez-Muñoz Jorge P, Cisneros Alejandro, Navarrete-Espinosa Joel, Sanchez-Sinencio Feliciano

机构信息

Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico.

Centro de Investigación en Micro y Nanotecnología (MICRONA), Universidad Veracruzana, Boca del Rio, Veracruz, 94294, Mexico.

出版信息

Sensors (Basel). 2014 Apr 25;14(5):7580-601. doi: 10.3390/s140507580.

DOI:10.3390/s140507580
PMID:24776933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4063023/
Abstract

Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.

摘要

在此，我们描述并测试了一种基于微阵列的登革病毒（DENV）血清型筛查方法。这种DNA微阵列检测方法具有特异性和灵敏性，能够检测出两种登革病毒血清型的双重感染以及单血清型感染。其他方法可能会低估含有多种血清型的样本。该技术可用于区分四种DENV血清型。单链DNA靶标被共价连接到载玻片上，并与特异性标记的探针杂交。使用DENV分离株和登革热样本评估微阵列性能。我们的结果表明，探针与DENV血清型特异性杂交，未检测到非特异性信号。这一发现证明特异性探针能够有效识别DENV样本中的单重和双重感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/decae99b573b/sensors-14-07580f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/007fde3af7ae/sensors-14-07580f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/b953b68c7206/sensors-14-07580f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/06c0804d38e4/sensors-14-07580f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/e7258c2ac36c/sensors-14-07580f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/decae99b573b/sensors-14-07580f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/007fde3af7ae/sensors-14-07580f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/b953b68c7206/sensors-14-07580f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/06c0804d38e4/sensors-14-07580f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/e7258c2ac36c/sensors-14-07580f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c8/4063023/decae99b573b/sensors-14-07580f5.jpg

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