HPV 9G DNAChip：基于 9G DNAChip 技术。

HPV 9G DNAChip: Based on the 9G DNAChip technology.

机构信息

Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Republic of Korea.

出版信息

J Virol Methods. 2012 Aug;183(2):132-8. doi: 10.1016/j.jviromet.2012.04.003. Epub 2012 Apr 25.

DOI:10.1016/j.jviromet.2012.04.003

PMID:22561985

Abstract

The novel HPV 9G DNAChips were developed for the detection and discrimination of the HPV genotypes in the clinical samples. The HPV 9G DNAChip established high SBR of 50-70 and 100% target-specific hybridization after 30min hybridization and 2×2min washing at 25°C. We compared the genotyping results of the 959 HPV positive and 82 HPV negative clinical samples by the HPV 9G DNAChip and the sequencing; the results are in 100% agreement. The HPV 9G DNAChip efficiently discriminate 19 HPV genotypes in the 959 HPV positive clinical samples. The results of HPV 9G DNAChip were 100% identical with the sequencing analysis in the detection and discrimination of HPV genotypes in the HPV negative clinical samples. The high SBR, 100% target-specific hybridization, and 25°C hybridization and washing makes the HPV 9G DNAChip a promising diagnostic tool for the accurate HPV genotyping.

摘要

新型 HPV 9G DNA 芯片用于检测和区分临床样本中的 HPV 基因型。HPV 9G DNA 芯片在 25°C 杂交 30 分钟和 2×2 分钟洗涤后，建立了 50-70 和 100%的高 SBR，具有高特异性杂交。我们比较了 HPV 9G DNA 芯片和测序对 959 例 HPV 阳性和 82 例 HPV 阴性临床样本的基因分型结果，结果完全一致。HPV 9G DNA 芯片可有效区分 959 例 HPV 阳性临床样本中的 19 种 HPV 基因型。HPV 9G DNA 芯片在 HPV 阴性临床样本中检测和区分 HPV 基因型的结果与测序分析完全一致。高 SBR、100%的靶特异性杂交以及 25°C 的杂交和洗涤使得 HPV 9G DNA 芯片成为一种有前途的 HPV 基因分型的准确诊断工具。