Division of Neurophysiology, Department of Physiology, Medical Faculty, University of Çukurova, 01330 Balcali, Adana, Turkey.
Behav Brain Res. 2012 Jul 15;233(1):157-61. doi: 10.1016/j.bbr.2012.04.041. Epub 2012 Apr 27.
N-methyl-D-aspartate (NMDA) receptors play an important role in brain maturation and developmental processes. In our study, we evaluated the effects of neonatal NMDA receptor blockade on exploratory locomotion and anxiety-like behaviors of adult BALB/c and C57BL/6 mice. In this study, NMDA receptor hypofunction was induced 7-10 days after birth using MK-801 in BALB/c and C57BL/6 mice (0.25mg/kg twice a day for 4 days via intraperitoneal injection). The open-field (OF) and elevated plus maze (EPM) tests were used to evaluate exploratory locomotion and anxiety-like behaviors. In the OF, BALB/c mice spent less time in the center of the field (p<0.05) and had less vertical locomotor activity (p<0.01) compared to C57BL/6 mice. In BALB/c mice, MK-801 caused a decrease in vertical and horizontal locomotor activity in the OF test, compared to the control group (p<0.05). In C57BL/6 mice, MK-801 treatment increased horizontal locomotor activity and decreased time spent in the center in the OF test (p<0.05). In the EPM, the number of open-arm entries, the percentage of open-arm time (p<0.01) and total arm entries (p<0.05) were lower in BALB/c mice compared to C57BL/6 mice. In BALB/c mice, MK-801 caused an increase in the percentage of open-arm time compared to the control group (p<0.05). In C57BL/6 mice, MK-801 caused a decrease in the percentage of open-arm time compared to the control group (p<0.05). MK-801 decreased exploratory and anxiety-like behaviors in BALB/c mice. In contrast, MK-801 increased exploratory and anxiety-like behaviors in C57BL/6 mice. In conclusion, hereditary factors may play an important role in neonatal NMDA receptor blockade-induced responses.
N-甲基-D-天冬氨酸（NMDA）受体在大脑成熟和发育过程中发挥重要作用。在我们的研究中，我们评估了新生 NMDA 受体阻断对 BALB/c 和 C57BL/6 小鼠成年期探索性运动和焦虑样行为的影响。在这项研究中，使用 MK-801 在 BALB/c 和 C57BL/6 小鼠中诱导 NMDA 受体功能低下，在出生后 7-10 天（通过腹腔内注射，每天两次，每次 0.25mg/kg，共 4 天）。使用旷场（OF）和高架十字迷宫（EPM）测试评估探索性运动和焦虑样行为。在 OF 中，与 C57BL/6 小鼠相比，BALB/c 小鼠在场中央停留的时间更少（p<0.05），垂直运动活动更少（p<0.01）。与对照组相比，MK-801 导致 BALB/c 小鼠 OF 测试中的垂直和水平运动活动减少（p<0.05）。在 C57BL/6 小鼠中，MK-801 处理增加了 OF 测试中的水平运动活动，并减少了在中央的时间（p<0.05）。在 EPM 中，与 C57BL/6 小鼠相比，BALB/c 小鼠进入开放臂的次数、开放臂时间的百分比（p<0.01）和总臂进入次数（p<0.05）较低。与对照组相比，MK-801 导致 BALB/c 小鼠的开放臂时间百分比增加（p<0.05）。与对照组相比，MK-801 导致 C57BL/6 小鼠的开放臂时间百分比降低（p<0.05）。MK-801 降低了 BALB/c 小鼠的探索性和焦虑样行为。相反，MK-801 增加了 C57BL/6 小鼠的探索性和焦虑样行为。总之，遗传因素可能在新生 NMDA 受体阻断诱导的反应中发挥重要作用。
