Division of Neurophysiology, Department of Physiology, Medical Faculty, University of Çukurova, 01330 Balcali, Adana, Turkey.
Pharmacol Biochem Behav. 2012 Jan;100(3):513-7. doi: 10.1016/j.pbb.2011.10.014. Epub 2011 Oct 20.
In this study, we evaluated the effect of ketamine on exploratory locomotion behaviours in the Balb/c and C57BL/6 strains of mice, which differ in their locomotion behaviours. Intraperitoneal administration of ketamine at three different doses (1, 5 or 10 mg/kg, 0.1 ml/10 gr body weight) was performed on adult male Balb/c and C57BL/6 mice. The same volume of saline was applied to the control group. The open-field and elevated plus maze apparatus were used to evaluate exploratory locomotion. In the open-field test, Balb/c mice less spend time in the centre of the field and was decreased locomotor activity compared to C57BL/6 mice (p<0.01). Ketamine treatment of Balb/c mice at 10 mg/kg dose caused an increase in locomotor activity and an increase in the amount of time spent in the centre in the open-field test, compared to the control group (p<0.05). In C57BL/6 mice, ketamine treatment (1 and 10 mg/kg) decreased locomotor activity (p<0.05). In C57BL/6 mice, the three different doses of ketamine application each caused a decrease in the frequency of centre crossing (p<0.001) and the spent time in the centre (p<0.05). In the elevated plus maze, the number of open-arm entries, the percentage of open-arm time and total arm entries were decreased in Balb/c mice compared to C57BL/6 mice (p<0.001). Ketamine treatment of Balb/c mice at 10 mg/kg dose caused an increase in the open-arm activity (p<0.001). Ketamine application (10 mg/kg) decreased the open-arm activity in C57BL/6 mice (p<0.05). A subanaesthetic dose of ketamine increased exploratory locomotion in Balb/c mice. In contrast, a subanaesthetic dose of ketamine decreased exploratory locomotion in C57BL/6 mice. In conclusion, hereditary factors may play an important role in ketamine-induced responses.
在这项研究中，我们评估了氯胺酮对不同运动行为的 Balb/c 和 C57BL/6 品系小鼠探索性运动行为的影响。氯胺酮以三种不同剂量（1、5 或 10 mg/kg，0.1 ml/10 gr 体重）腹腔给药于成年雄性 Balb/c 和 C57BL/6 小鼠。对照组给予相同体积的生理盐水。使用旷场和高架十字迷宫装置评估探索性运动。在旷场测试中，Balb/c 小鼠在中央区域停留的时间较少，与 C57BL/6 小鼠相比，运动活性降低（p<0.01）。与对照组相比，氯胺酮治疗 Balb/c 小鼠 10 mg/kg 剂量导致运动活性增加，并且在旷场测试中在中央区域花费的时间增加（p<0.05）。在 C57BL/6 小鼠中，氯胺酮治疗（1 和 10 mg/kg）降低了运动活性（p<0.05）。在 C57BL/6 小鼠中，三种不同剂量的氯胺酮应用均导致中央穿越频率降低（p<0.001）和中央停留时间减少（p<0.05）。在高架十字迷宫中，与 C57BL/6 小鼠相比，Balb/c 小鼠进入开放臂的次数、开放臂时间的百分比和总臂进入次数减少（p<0.001）。氯胺酮治疗 Balb/c 小鼠 10 mg/kg 剂量导致开放臂活动增加（p<0.001）。氯胺酮应用（10 mg/kg）降低了 C57BL/6 小鼠的开放臂活动（p<0.05）。亚麻醉剂量的氯胺酮增加了 Balb/c 小鼠的探索性运动。相反，亚麻醉剂量的氯胺酮降低了 C57BL/6 小鼠的探索性运动。总之，遗传因素可能在氯胺酮诱导的反应中发挥重要作用。
