Department of Medical Microbiology and Parasitology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
Parasitol Res. 2012 Sep;111(3):1137-42. doi: 10.1007/s00436-012-2945-3. Epub 2012 May 6.
A few PCR-based DNA typing methods using repetitive elements contained within both protein-coding genes and noncoding DNAs have been reported for Entamoeba histolytica over the years. The serine-rich E. histolytica protein and tRNA-linked short tandem repeats (STRs) are most commonly used to investigate the relationship between parasite genotype and E. histolytica infection outcome. Many E. histolytica infections in China have been reported; however, little genome information has been provided. In the current paper, five Chinese E. histolytica samples were reported: three amoebic liver abscess cases, one combined case and one asymptomatic case. Our study is the first to report on the DNA typing information of E. histolytica in China. We included two city, one imported, and two country cases. Sequence analysis of serine-rich protein genes confirmed the presence of seven sequence types in five isolates. The STRs amplified from the samples revealed five STR variations in the A-L, four in the N-K2, and R-R loci, three in D-A, S(TGA)-D and S-Q loci. Two country patients were found to have a different outcome of infection with the same genotypes of E. histolytica, whereas in a city case, one E. histolytica strain had led to different outcome of the infection in one patient. Analyses of the results suggest that more genome information of E. histolytica strains from China through accurate methods is needed to interpret how the parasite genome plays a role in determining the outcome of E. histolytica infections.
多年来，已有一些基于聚合酶链反应（PCR）的 DNA 分型方法被报道用于检测内阿米巴原虫（Entamoeba histolytica），这些方法利用了编码蛋白基因和非编码 DNA 中包含的重复元件。富含丝氨酸的内阿米巴原虫蛋白和 tRNA 连接的短串联重复序列（STRs）是最常用于研究寄生虫基因型与内阿米巴原虫感染结果之间关系的方法。中国已报道了许多内阿米巴原虫感染病例，但提供的基因组信息较少。在本研究中，报道了 5 株中国分离的内阿米巴原虫：3 例阿米巴肝脓肿、1 例混合感染和 1 例无症状感染。本研究是首次报道中国内阿米巴原虫的 DNA 分型信息。我们包括了 2 个城市、1 个输入性和 2 个农村病例。对富含丝氨酸蛋白基因的序列分析证实，在 5 个分离株中存在 7 种序列类型。从样本中扩增的 STRs 显示 A-L 位点有 5 种 STR 变异，N-K2 和 R-R 位点有 4 种，D-A、S(TGA)-D 和 S-Q 位点有 3 种。2 例农村患者感染了相同基因型的内阿米巴原虫，但感染结果不同，而在城市病例中，同一患者感染了不同的内阿米巴原虫株。结果分析表明，需要通过准确的方法获得更多中国内阿米巴原虫株的基因组信息，以解释寄生虫基因组如何在确定内阿米巴原虫感染结果中发挥作用。
