Department of Biotechnology, Shivaji University, Kolhapur 416004, India.
Environ Sci Pollut Res Int. 2013 Feb;20(2):1009-20. doi: 10.1007/s11356-012-0929-6. Epub 2012 May 5.
Bioremediation of textile dyestuffs under solid-state fermentation (SSF) using industrial wastes as substrate pose an economically feasible, promising, and eco-friendly alternative. The purpose of this study was to adsorb Red M5B dye, a sample of dyes mixture and a real textile effluent on distillery industry waste-yeast biomass (DIW-YB) and its further bioremediation using Bacillus cereus EBT1 under SSF. Textile dyestuffs were allowed to adsorb on DIW-YB. DIW-YB adsorbed dyestuffs were decolorized under SSF by using B. cereus. Enzyme analysis was carried out to ensure decolorization of Red M5B. Metabolites after dye degradation were analyzed using UV-Vis spectroscopy, FTIR, HPLC, and GC-MS. DIW-YB showed adsorption of Red M5B, dyes mixture and a textile wastewater sample up to 87, 70, and 81 %, respectively. DIW-YB adsorbed Red M5B was decolorized up to 98 % by B. cereus in 36 h. Whereas B. cereus could effectively reduce American Dye Manufacture Institute value from DIW-YB adsorbed mixture of textile dyes and textile wastewater up to 70 and 100 %, respectively. Induction of extracellular enzymes such as laccase and azoreductase suggests their involvement in dye degradation. Repeated utilization of DIW-YB showed consistent adsorption and ADMI removal from textile wastewater up to seven cycles. HPLC and FTIR analysis confirms the biodegradation of Red M5B. GC-MS analysis revealed the formation of new metabolites. B. cereus has potential to bioremediate adsorbed textile dyestuffs on DIW-YB. B. cereus along with DIW-YB showed enhanced decolorization performance in tray bioreactor which suggests its potential for large-scale treatment procedures.
采用工业废料作为基质进行固态发酵（SSF）生物修复纺织染料具有经济可行、有前途和环保的替代方案。本研究旨在利用酿酒工业废料-酵母生物质（DIW-YB）吸附 Red M5B 染料、染料混合物样品和实际纺织废水，并在 SSF 条件下用蜡状芽孢杆菌 EBT1 进一步进行生物修复。让纺织染料吸附在 DIW-YB 上。在 SSF 条件下，使用蜡状芽孢杆菌使 DIW-YB 吸附的染料脱色。进行酶分析以确保 Red M5B 脱色。使用 UV-Vis 光谱、FTIR、HPLC 和 GC-MS 分析染料降解后的代谢物。DIW-YB 对 Red M5B、染料混合物和纺织废水样品的吸附率分别达到 87%、70%和 81%。蜡状芽孢杆菌可在 36 小时内将 DIW-YB 吸附的 Red M5B 脱色 98%。蜡状芽孢杆菌可有效降低 DIW-YB 吸附的纺织染料混合物和纺织废水中的美国染料制造商协会值，分别达到 70%和 100%。诱导细胞外酶如漆酶和偶氮还原酶表明它们参与了染料降解。重复利用 DIW-YB 从纺织废水中显示出一致的吸附和 ADMI 去除，可达 7 个循环。HPLC 和 FTIR 分析证实了 Red M5B 的生物降解。GC-MS 分析表明形成了新的代谢物。蜡状芽孢杆菌具有在 DIW-YB 上生物修复吸附的纺织染料的潜力。蜡状芽孢杆菌和 DIW-YB 在托盘生物反应器中表现出增强的脱色性能，表明其在大规模处理程序中的潜力。
