Borham Ali, Okla Mohammad K, El-Tayeb Mohamed A, Gharib Ahmed, Hafiz Hanan, Liu Lei, Zhao Chen, Xie Ruqing, He Nannan, Zhang Siwen, Wang Juanjuan, Qian Xiaoqing
Key Laboratory of Cultivated Land Quality Monitoring and Evaluation, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225127, China.
Agriculture Products Safety and Environment, College of Agriculture, Yangzhou University, Yangzhou 225127, China.
J Fungi (Basel). 2023 Nov 1;9(11):1069. doi: 10.3390/jof9111069.
Textile dyes are one of the major water pollutants released into water in various ways, posing serious hazards for both aquatic organisms and human beings. Bioremediation is a significantly promising technique for dye decolorization. In the present study, the fungal strain sp. was isolated from the fruiting bodies of for the first time. The isolated fungal strain was examined for laccase enzyme production under solid-state fermentation conditions with wheat bran (WB) using ABTS and 2,6-Dimethoxyphenol (DMP) as substrates, then the fermented wheat bran (FWB) was evaluated as a biosorbent for Congo red dye adsorption from aqueous solutions in comparison with unfermented wheat bran. A Box-Behnken design was used to optimize the dye removal by FWB and to analyze the interaction effects between three factors: fermentation duration, pH, and dye concentration. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) were applied to study the changes in the physical and chemical characteristics of wheat bran before and after fermentation. An additional experiment was conducted to investigate the ability of the sp. YZH1 to remove Congo red in the dye-containing liquid culture. The results showed that laccase was produced throughout the cultivation, reaching peak activities of ∼6.2 and 22.3 U/mL for ABTS and DMP, respectively, on the fourth day of cultivation. FWB removed 89.8% of the dye (100 mg L) from the aqueous solution after 12 h of contact, whereas WB removed only 77.5%. Based on the Box-Behnken design results, FWB achieved 93.08% dye removal percentage under the conditions of 6 days of fermentation, pH 8.5, and 150 mg L of the dye concentration after 24 h. The fungal strain removed 95.3% of 150 mg L of the dye concentration after 8 days of inoculation in the dye-containing liquid culture. These findings indicate that this strain is a worthy candidate for dye removal from environmental effluents.
纺织染料是通过多种方式排放到水中的主要水污染物之一，对水生生物和人类都构成严重危害。生物修复是一种极具前景的染料脱色技术。在本研究中，首次从 的子实体中分离出真菌菌株 sp.。在固态发酵条件下，以麦麸（WB）为培养基，使用ABTS和2,6 - 二甲氧基苯酚（DMP）作为底物，检测分离出的真菌菌株产漆酶的情况，然后将发酵后的麦麸（FWB）与未发酵的麦麸进行比较，评估其作为生物吸附剂从水溶液中吸附刚果红染料的性能。采用Box - Behnken设计优化FWB对染料的去除效果，并分析发酵时间、pH值和染料浓度这三个因素之间的相互作用。应用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和扫描电子显微镜（SEM）研究发酵前后麦麸的物理和化学特性变化。进行了一项额外实验，以研究 sp. YZH1在含染料液体培养物中去除刚果红的能力。结果表明，在整个培养过程中都产生了漆酶，在培养的第四天，以ABTS和DMP为底物时，漆酶活性分别达到约6.2和22.3 U/mL的峰值。接触12小时后，FWB从水溶液中去除了89.8%的染料（100 mg/L），而WB仅去除了77.5%。根据Box - Behnken设计结果，在发酵6天、pH值8.5和染料浓度150 mg/L的条件下，24小时后FWB对染料的去除率达到93.08%。在含染料液体培养物中接种8天后，该真菌菌株去除了150 mg/L染料浓度的95.3%。这些发现表明，该菌株是从环境废水中去除染料的一个有价值的候选菌株。
