State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095 Jiangsu, China.
Mol Biol Rep. 2012 Aug;39(8):8147-58. doi: 10.1007/s11033-012-1662-4. Epub 2012 May 6.
Chickpea (Cicer arietinum L.) is an important pulse crop grown mainly in the arid and semi-arid regions of the world. Due to its taxonomic proximity with the model legume Medicago truncatula and its ability to grow in arid soil, chickpea has its unique advantage to understand how plant responds to drought stress. In this study, an oligonucleotide microarray was used for analyzing the transcriptomic profiles of unigenes in leaf and root of chickpea seedling under drought stress, respectively. Microarray data showed that 4,815 differentially expressed unigenes were either ≥ 2-fold up- or ≤ 0.5-fold down-regulated in at least one of the five time points during drought stress. 2,623 and 3,969 unigenes were time-dependent differentially expressed in root and leaf, respectively. 110 pathways in two tissues were found to respond to drought stress. Compared to control, 88 and 52 unigenes were expressed only in drought-stressed root and leaf, respectively, while nine unigenes were expressed in both the tissues. 1,922 function-unknown unigenes were found to be remarkably regulated by drought stress. The expression profiles of these time-dependent differentially expressed unigenes were useful in furthering our knowledge of molecular mechanism of plant in response to drought stress.
鹰嘴豆（Cicer arietinum L.）是一种重要的豆类作物，主要生长在世界的干旱和半干旱地区。由于其与模式豆科植物紫花苜蓿在分类学上的亲缘关系，以及其在干旱土壤中生长的能力，鹰嘴豆在研究植物如何应对干旱胁迫方面具有独特的优势。在这项研究中，我们使用寡核苷酸微阵列分析了鹰嘴豆幼苗叶片和根系在干旱胁迫下的转录组谱。微阵列数据显示，在干旱胁迫的五个时间点中的至少一个时间点，有 4815 个差异表达的基因要么上调 2 倍以上，要么下调 0.5 倍以下。在根和叶中，有 2623 和 3969 个基因分别表现出时间依赖性差异表达。在两个组织中发现了 110 条对干旱胁迫有反应的途径。与对照相比，在干旱胁迫的根和叶中分别有 88 和 52 个基因只在受胁迫的组织中表达，而 9 个基因在两个组织中都表达。发现有 1922 个功能未知的基因受到干旱胁迫的显著调控。这些时间依赖性差异表达基因的表达谱有助于进一步了解植物对干旱胁迫的分子机制。
