Analytical Biosensors Group (GBA), Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, 50009, Zaragoza, Spain.
Anal Bioanal Chem. 2012 Aug;404(2):351-9. doi: 10.1007/s00216-012-6061-0. Epub 2012 May 5.
In the context of sustainable analytical chemistry, phenol has been determined through its enzymatic reaction with laccase. The method has been studied and optimized through the autoindicating optical properties of laccase both by intrinsic molecular absorption and fluorescence. The method shows a linear range from 9.79·10(-6) to 7.50·10(-4) M with a relative standard deviation of 1.07 %. The molecular absorption methodology has been implemented in a polyacrylamide film for the design of an autoindicating optical sensor. In order to increase the lifetime of the sensor, the reversibility study of the enzymatic reaction has proposed, as a novelty, the regeneration of laccase with an oxidase-type enzyme (glucose oxidase). The lifetime of the sensor film has improved from 15 to 30 measurements. The reaction mechanism has also been studied and confirmed by fluorescence and molecular absorption. The method leads to the determination of phenol in environmental samples.
在可持续分析化学的背景下，通过酚与漆酶的酶促反应来测定酚。该方法通过漆酶的固有分子吸收和荧光的自动指示光学特性进行了研究和优化。该方法的线性范围为 9.79·10(-6) 至 7.50·10(-4) M，相对标准偏差为 1.07%。分子吸收方法已在聚丙烯酰胺薄膜中实施，用于设计自动指示光学传感器。为了提高传感器的寿命，作为一项创新，提出了用氧化酶型酶（葡萄糖氧化酶）来再生漆酶，以提高传感器的寿命。酶反应的可逆性研究。传感器膜的寿命从 15 次测量提高到 30 次测量。还通过荧光和分子吸收研究并证实了反应机理。该方法可用于测定环境样品中的苯酚。
