Department of Gastrointestinal Surgery, Institute of Digestive Surgery and National Key Laboratory of Biotherapy of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.
Med Oncol. 2012 Dec;29(5):3113-8. doi: 10.1007/s12032-012-0241-9. Epub 2012 May 5.
The recently identified class of microRNAs (miRNAs) provided a new insight in cancer research. As the member of miRNAs family, miR-34a, miR-155 and miR-200c abnormalities have been found in various types of cancer. However, the relationship between these three miRNAs (miR-34a, miR-155 and miR-200c) and colorectal cancer is unclear. In this study, we applied stem-loop real-time PCR to quantitatively detect miR-34a, miR-155 and miR-200c expression in 109 pair-matched human colorectal cancers and the corresponding normal mucosa. MiR-34a (2.2-fold), miR-155 (2.3-fold) and miR-200c (3.1-fold) were all expressed at higher levels in colorectal cancer (P = 0.001, 0.005 and 0.001, respectively). In rectum, miR-34a and miR-200c were significantly upregulated (P = 0.006 and 0.007), while the miR-155 overexpression was not statistically significant (P = 0.083). In colon, the higher expression of three miRNAs was seen, however, without significant difference (P > 0.05). We also found that the miR-34a expression was higher in rectal cancer having more advanced TNM stage (III + IV, P = 0.03). Then miR-200c expression was positively correlated with and sera CEA level of rectal cancer patients (P = 0.04). In conclusion, our results thus suggest that the overexpression of miR-34a, miR-155 and miR-200c be associated with the development of colorectal cancer, meanwhile miR-34a may be involved in the development and progression of rectal cancer. The more deeply and larger scale research are required to prove the correlation.
最近发现的一类 microRNAs（miRNAs）为癌症研究提供了新的视角。miR-34a、miR-155 和 miR-200c 作为 miRNAs 家族的成员，已在各种类型的癌症中发现存在异常。然而，这三种 miRNAs（miR-34a、miR-155 和 miR-200c）与结直肠癌之间的关系尚不清楚。在这项研究中，我们应用茎环实时 PCR 定量检测了 109 对配对的人结直肠癌组织及其相应的正常黏膜中 miR-34a、miR-155 和 miR-200c 的表达。miR-34a（2.2 倍）、miR-155（2.3 倍）和 miR-200c（3.1 倍）在结直肠癌中表达水平均升高（P=0.001、0.005 和 0.001）。在直肠中，miR-34a 和 miR-200c 明显上调（P=0.006 和 0.007），而 miR-155 的过表达无统计学意义（P=0.083）。在结肠中，三种 miRNAs 的高表达，但无显著差异（P>0.05）。我们还发现 miR-34a 在 TNM 分期较高（III+IV）的直肠癌症中表达较高（P=0.03）。然后 miR-200c 的表达与直肠癌患者的血清 CEA 水平呈正相关（P=0.04）。总之，我们的结果表明 miR-34a、miR-155 和 miR-200c 的高表达与结直肠癌的发生有关，同时 miR-34a 可能参与直肠癌症的发生和进展。需要进行更深入和更大规模的研究来证明这种相关性。
