Department of Computer Science, University of California, Riverside, CA 92521, USA.
Bioinformatics. 2012 Jul 1;28(13):1795-6. doi: 10.1093/bioinformatics/bts264. Epub 2012 May 3.
We introduce BRAT-BW, a fast, accurate and memory-efficient tool that maps bisulfite-treated short reads (BS-seq) to a reference genome using the FM-index (Burrows-Wheeler transform). BRAT-BW is significantly more memory efficient and faster on longer reads than current state-of-the-art tools for BS-seq data, without compromising on accuracy. BRAT-BW is a part of a software suite for genome-wide single base-resolution methylation data analysis that supports single and paired-end reads and includes a tool for estimation of methylation level at each cytosine.
The software is available in the public domain at http://compbio.cs.ucr.edu/brat/.
我们介绍了 BRAT-BW,这是一款快速、准确且内存效率高的工具,它使用 FM-index(Burrows-Wheeler 变换)将经亚硫酸氢盐处理的短读取(BS-seq)映射到参考基因组上。BRAT-BW 在处理较长读取时比当前最先进的 BS-seq 数据工具更节省内存且速度更快,同时在准确性上也没有妥协。BRAT-BW 是一套用于全基因组单碱基分辨率甲基化数据分析的软件套件的一部分,它支持单端和双端读取,并包括一个用于估计每个胞嘧啶甲基化水平的工具。
该软件可在公共领域通过 http://compbio.cs.ucr.edu/brat/ 获取。