Laboratório de Estresse Oxidativo e Poluição Atmosférica, Universidade Federal de Ciências da Saúde de Porto Alegre, Brasil.
Toxicol Mech Methods. 2012 Sep;22(7):533-9. doi: 10.3109/15376516.2012.692109. Epub 2012 May 30.
The current study aimed to determine the role of oxidants in cardiac and pulmonary toxicities induced by chronic exposure to ROFA. Eighty Wistar rats were divided into four groups: G1 (10 µL Saline), G2 (ROFA 50 µg/10 µL), G3 (ROFA 250 µg/10 µL) and G4 (ROFA 500 µg/10 µL). Rats received ROFA by nasotropic instillation for 90 days. After that, they were euthanized and bronchoalveolar lavage (BAL) was performed for total count of leukocytes, protein and lactate dehydrogenase (LDH) determinations. Lungs and heart were removed to measure lipid peroxidation (MDA), catalase (CAT) and superoxide dismutase (SOD) activity. BAL presented an increase in leukocytes count in G4 in comparison to the Saline group (p = 0.019). In lung, MDA level was not modified by ROFA, while CAT was higher in G4 when compared to all other groups (p = 0.013). In heart, G4 presented an increase in MDA (p = 0.016) and CAT (p = 0.027) levels in comparison to G1. The present study demonstrated cardiopulmonary oxidative changes after a chronic ROFA exposure. More specifically, the heart tissue seems to be more susceptible to oxidative effects of long-term exposure to ROFA than the lung.
本研究旨在确定氧化剂在慢性暴露于 ROFA 引起的心脏和肺毒性中的作用。80 只 Wistar 大鼠分为四组：G1（10 µL 生理盐水）、G2（ROFA 50 µg/10 µL）、G3（ROFA 250 µg/10 µL）和 G4（ROFA 500 µg/10 µL）。大鼠通过鼻腔滴注 ROFA 90 天。之后，处死大鼠并进行支气管肺泡灌洗（BAL），以测定白细胞总数、蛋白和乳酸脱氢酶（LDH）。取出肺和心脏以测量脂质过氧化（MDA）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性。BAL 显示 G4 组白细胞计数高于生理盐水组（p = 0.019）。在肺中，ROFA 并未改变 MDA 水平，而 G4 组的 CAT 水平高于其他所有组（p = 0.013）。在心脏中，G4 组的 MDA（p = 0.016）和 CAT（p = 0.027）水平均高于 G1 组。本研究表明，慢性 ROFA 暴露后会引起心肺氧化变化。更具体地说，与肺相比，心脏组织似乎更容易受到长期暴露于 ROFA 的氧化作用影响。
