Coballase-Urrutia Elvia, Cárdenas-Rodríguez Noemí, González-García María Carolina, Núñez-Ramírez Eithan, Floriano-Sánchez Esaú, González-Trujano María Eva, Fernández-Rojas Berenice, Pedraza-Chaverrí José, Montesinos-Correa Hortencia, Rivera-Espinosa Liliana, Sampieri Aristides Iii, Carmona-Aparicio Liliana
Laboratory of Neuroscience, National Institute of Pediatrics, 04530 D.F. Mexico, Mexico.
Military School of Graduate of Health, Multidisciplinary Research Laboratory, SEDENA, 11270 D.F. Mexico, Mexico.
Saudi Pharm J. 2017 Mar;25(3):319-331. doi: 10.1016/j.jsps.2016.06.008. Epub 2016 Jul 12.
Around the world, species from the genus are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous extracts (100 mg/kg, i.p.) obtained from the leaves of var. on CCl-induced liver and brain damage in the rat. Protection was observed in the liver and brain (cerebellum, cortex and cerebral hemispheres) by measuring the activity of antioxidant enzymes and levels of malondialdehyde (MDA) using spectrophotometric methods. Biochemical parameters were also assessed in serum samples from the CCl-treated rats. The var. leaf extracts provided significant protection against CCl-induced peripheral and central damage by increasing the activity of antioxidant enzymes, diminishing lipid peroxidation, and preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-globulin (γ-GLOB), serum albumin (ALB), total bilirubin (BB), creatinine (CREA) and creatine kinase (CK), relative to the control group. Additionally, we correlated gene expression with antioxidant activity in the experimental groups treated with the organic and aqueous extracts and observed a non-statistically significant positive correlation. Our results provide evidence of the underlying biomedical properties of var. that confer its neuro- and hepatoprotective effects.
在世界各地，该属的物种因其外周和中枢药用作用而被广泛使用；它们被制成茶剂，用作镇静、抗惊厥和止痛剂。在本研究中，我们提供了从某变种的叶子中获得的有机提取物和水提取物（100毫克/千克，腹腔注射）对大鼠四氯化碳诱导的肝损伤和脑损伤具有保护作用的证据。通过分光光度法测量抗氧化酶的活性和丙二醛（MDA）的水平，在肝脏和大脑（小脑、皮层和大脑半球）中观察到了保护作用。还对四氯化碳处理大鼠的血清样本中的生化参数进行了评估。相对于对照组，该变种叶提取物通过提高抗氧化酶的活性、减少脂质过氧化以及防止生化血清参数（如天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、γ-球蛋白（γ-GLOB）、血清白蛋白（ALB）、总胆红素（BB）、肌酐（CREA）和肌酸激酶（CK））的改变，对四氯化碳诱导的外周和中枢损伤提供了显著保护。此外，我们将基因表达与用有机提取物和水提取物处理的实验组中的抗氧化活性进行了关联，并观察到了无统计学意义的正相关。我们的结果提供了该变种具有潜在生物医学特性的证据，这些特性赋予了其神经保护和肝保护作用。
