Chen T, Cheng M, Yuan Z, Zhou S, Yu Z
Department of general surgery, Yong Chuan affiliated hospital of Chongqing Medical University, Chongqing, PR. China.
Transplant Proc. 2012 May;44(4):978-81. doi: 10.1016/j.transproceed.2012.03.052.
We sought to investigate the protective role of Shenfu (SF),a traditional Chinese formulation comprising Radix Ginseng and Radix Aconitum Carmichaeli on ischemia-reperfusion injury in rat liver grafts.
Ninety-six male Sprague Dawley rats were used as donors (n = 48) and recipients (n = 48) of orthotopic liver transplantation. They were randomly divided into a control group with donor livers injected with saline through the portal vein immediately after recovery versus the SF group, with livers injected with SF. Each group was further divided into 3 subgroups equally to obtain bood and hepatic tissues samples at 2, 4, and 6 hours reperfusion.
At each phase, the SF group, showed significantly higher bile production (P < .05) with lower serum levels of alanine aminotransferase and tumor necrosis factor-α, and nuclear factor-κB expression in the hepatic tissues. (P < .05). SF group hepatic tissues showed less injury compared with controls.
SF injection seemed to protect hepatocytes from injury during the early reperfusion phase and to improve subsequent rat liver graft function.
我们试图研究参附（SF）（一种由人参和附子组成的传统中药配方）对大鼠肝移植缺血再灌注损伤的保护作用。
96只雄性Sprague Dawley大鼠用作原位肝移植的供体（n = 48）和受体（n = 48）。它们被随机分为对照组（供体肝脏在恢复后立即通过门静脉注射生理盐水）和SF组（肝脏注射SF）。每组再平均分为3个亚组，在再灌注2、4和6小时时获取血液和肝组织样本。
在每个阶段，SF组胆汁分泌显著更高（P < 0.05），血清丙氨酸转氨酶水平和肿瘤坏死因子-α以及肝组织中核因子-κB表达更低（P < 0.05）。与对照组相比，SF组肝组织损伤更小。
注射SF似乎在再灌注早期阶段保护肝细胞免受损伤，并改善后续大鼠肝移植功能。
