Lin C M, Lee J F, Chiang L L, Chen C F, Wang D, Su C L
Division of Pulmonary Medicine, Department of Internal Medicine, Shin Kong Wu-Ho-Su Memorial Hospital, Taipei, Taiwan.
Transplant Proc. 2012 May;44(4):974-7. doi: 10.1016/j.transproceed.2012.01.081.
Reperfusion of the ischemic liver results in the generation of oxidative and nitrosative stresses and reaction product of peroxynitrite, which induce rapid cytotoxicity and liver injury. In this study we demonstrated that curcumin, an antioxidant, attenuated ischemia/reperfusion (I/R)-induced liver injury.
Ischemia was induced by clamping the common hepatic artery and portal vein of rats for 30 minutes. Thereafter, flow was restored and the liver was reperfused for 80 minutes. Blood samples collected prior to ischemia and after reperfusion were analyzed for methyl guanidine (MG), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and adenosphate triphosphate (ATP). Blood levels of serum glutamic oxaloacetic transaminase (sGOT), serum glutamate pyruvate transaminase (sGPT), and lactic dehydrogenase (LDH), which served as indexes of liver injury, were measured.
The protocol resulted in elevation of blood NO (P < .001), TNF-α (P < .001), and MG (P < .001). sGOT, sGPT, and LDH were elevated significantly (P < .001), whereas ATP was significantly diminished (P < .001). Pretreatment with curcumin (25 mg/kg) significantly attenuated the reperfusion liver injury, while the ATP content reversed. In addition, MG, TNF-α, and NO release were attenuated.
These results indicated that curcumin exerted potent anti-inflammatory effects in I/R-induced liver injury due to its antioxidant effects.
缺血肝脏再灌注会导致氧化应激、亚硝化应激以及过氧亚硝酸盐反应产物的生成，进而引发快速的细胞毒性和肝损伤。在本研究中，我们证明了抗氧化剂姜黄素可减轻缺血/再灌注（I/R）诱导的肝损伤。
通过夹闭大鼠肝总动脉和门静脉30分钟诱导缺血。此后，恢复血流并使肝脏再灌注80分钟。对缺血前和再灌注后采集的血样进行甲基胍（MG）、一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和三磷酸腺苷（ATP）分析。测量作为肝损伤指标的血清谷氨酸草酰乙酸转氨酶（sGOT）、血清谷氨酸丙酮酸转氨酶（sGPT）和乳酸脱氢酶（LDH）的血中水平。
该方案导致血中NO（P <.001）、TNF-α（P <.001）和MG（P <.001）升高。sGOT、sGPT和LDH显著升高（P <.001），而ATP显著减少（P <.001）。用姜黄素（25 mg/kg）预处理可显著减轻再灌注肝损伤，同时ATP含量恢复。此外，MG、TNF-α和NO释放减少。
这些结果表明，姜黄素因其抗氧化作用在I/R诱导的肝损伤中发挥了强大的抗炎作用。
