Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, 030018, Bucharest, Romania.
Bioprocess Biosyst Eng. 2012 Nov;35(9):1523-30. doi: 10.1007/s00449-012-0742-0. Epub 2012 May 8.
The effect of water-alcohol (methanol, ethanol, propan-1-ol, propan-2-ol, ethane-1,2-diol and propane-1,2,3-triol) binary mixtures on the kinetics of hydrogen peroxide decomposition in the presence of bovine liver catalase is investigated. In all solvents, the activity of catalase is smaller than in water. The results are discussed on the basis of a simple kinetic model. The kinetic constants for product formation through enzyme-substrate complex decomposition and for inactivation of catalase are estimated. The organic solvents are characterized by several physical properties: dielectric constant (D), hydrophobicity (log P), concentration of hydroxyl groups ([OH]), polarizability (α), Kamlet-Taft parameter (β) and Kosower parameter (Z). The relationships between the initial rate, kinetic constants and medium properties are analyzed by linear and multiple linear regression.
研究了水-醇（甲醇、乙醇、正丙醇、异丙醇、乙烷-1,2-二醇和丙烷-1,2,3-三醇）二元混合物对牛肝过氧化氢酶存在下过氧化氢分解动力学的影响。在所有溶剂中，过氧化氢酶的活性均小于水。结果基于简单的动力学模型进行了讨论。通过酶-底物复合物分解和过氧化氢酶失活来估计产物形成的动力学常数。有机溶剂的性质用介电常数（D）、疏水性（log P）、羟基浓度([OH])、极化率（α）、Kamlet-Taft 参数（β）和 Kosower 参数（Z）来表征。通过线性和多元线性回归分析了初始速率、动力学常数和介质性质之间的关系。
