Tsai Y T, Chen S H, Lin K L, Hsieh K H
Department of Pediatrics, National Taiwan University, Taipei, Republic of China.
Ann Allergy. 1990 Dec;65(6):459-62.
A panel of tests including intracutaneous skin testing (ST), radioallergosorbent test (RAST), immunoblotting and allergen-induced lymphoproliferation was done to study rice pollen allergy in asthmatic children and to characterize the allergens. Of the 312 asthmatic patients skin tested, 29 (9.3%) had positive reactions (wheal greater than or equal to 6 mm) to rice pollen extract at a concentration of 10(-5) g/mL and the remaining 283 (90.7%) were negative. While eight (34.8%) of the 23 ST-positive patients were also RAST-positive, RAST was negative in all 34 ST-negative patients and 20 normals. Immunoblotting revealed three major allergens, with molecular weights of 16 kD, 26 kD, and 32 kD, respectively. Interestingly, RAST-positive patients showed IgE responses to most allergens but only a few of them had IgG antibodies, while normal controls had stronger IgG responses to the same allergens, particularly to 32 kD, but none had IgE antibody. The preliminary results of rice pollen protein induced-lymphoproliferation were not informative; thus, rice pollen proteins do elicit a specific response in asthmatic children and normals, but its pathogenic role in bronchial asthma needs further study.
为研究哮喘儿童的水稻花粉过敏情况并对过敏原进行特征分析，进行了一组包括皮内皮肤试验（ST）、放射变应原吸附试验（RAST）、免疫印迹法和过敏原诱导的淋巴细胞增殖试验。在接受皮肤试验的312例哮喘患者中，29例（9.3%）对浓度为10（-5）g/mL的水稻花粉提取物有阳性反应（风团大于或等于6mm），其余283例（90.7%）为阴性。在23例ST阳性患者中，8例（34.8%）RAST也呈阳性，而在所有34例ST阴性患者和20例正常人中RAST均为阴性。免疫印迹法显示有三种主要过敏原，分子量分别为16kD、26kD和32kD。有趣的是，RAST阳性患者对大多数过敏原显示IgE反应，但其中只有少数人有IgG抗体，而正常对照对相同过敏原的IgG反应更强，尤其是对32kD的过敏原，但没有人有IgE抗体。水稻花粉蛋白诱导的淋巴细胞增殖的初步结果没有提供有用信息；因此，水稻花粉蛋白确实在哮喘儿童和正常人中引发了特异性反应，但其在支气管哮喘中的致病作用需要进一步研究。
