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通过体细胞核移植生产转基因猪和基因敲除猪。

Production of transgenic and knockout pigs by somatic cell nuclear transfer.

作者信息

Giraldo Angelica M, Ball Suyapa, Bondioli Kenneth R

机构信息

Revivicor, Inc., Blacksburg, VA, USA.

出版信息

Methods Mol Biol. 2012;885:105-23. doi: 10.1007/978-1-61779-845-0_8.

DOI:10.1007/978-1-61779-845-0_8
PMID:22565993
Abstract

Xenotransplantation is one alternative to transplantation of human organs which has been investigated. It is generally accepted that the pig represents the most logical choice of animals to serve as organ donors for xenotransplantation. Moreover, the implementation of cloning by somatic cell nuclear transfer (SCNT) and transgenic techniques have resulted in the production of numerous transgenic pigs than can be used for xenotransplantation purposes as well as models for human diseases.

摘要

异种移植是已被研究的人类器官移植的一种替代方法。人们普遍认为,猪是作为异种移植器官供体的最合理动物选择。此外,体细胞核移植(SCNT)和转基因技术的应用已培育出大量可用于异种移植及人类疾病模型的转基因猪。

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1
Production of transgenic and knockout pigs by somatic cell nuclear transfer.通过体细胞核移植生产转基因猪和基因敲除猪。
Methods Mol Biol. 2012;885:105-23. doi: 10.1007/978-1-61779-845-0_8.
2
Development of the techniques for nuclear transfer in pigs.猪细胞核移植技术的发展。
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Establishment of an efficient somatic cell nuclear transfer system for production of transgenic pigs.建立高效的体细胞核移植系统生产转基因猪。
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Nuclear transfer in nonhuman primates.非人灵长类动物的核移植
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Production of cloned NIBS (Nippon Institute for Biological Science) and α-1, 3-galactosyltransferase knockout MGH miniature pigs by somatic cell nuclear transfer using the NIBS breed as surrogates.利用日本生物科学研究所(NIBS)品种作为代孕,通过体细胞核移植生产克隆的 NIBS 和α-1,3-半乳糖基转移酶敲除 MGH 小型猪。
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Production of dairy goat embryos, by nuclear transfer, transgenic for human acid beta-glucosidase.生产乳用山羊胚胎,通过核转移技术,使其转基因表达人酸性β-葡萄糖苷酶。
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The perspectives for porcine-to-human xenografts.猪到人的异种移植前景。
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Combined Chaetocin/Trichostatin A Treatment Improves the Epigenetic Modification and Developmental Competence of Porcine Somatic Cell Nuclear Transfer Embryos.联合使用茶托菌素/曲古抑菌素A处理可改善猪体细胞核移植胚胎的表观遗传修饰和发育能力。
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3
Humanized von Willebrand factor reduces platelet sequestration in ex vivo and in vivo xenotransplant models.
人源化血管性血友病因子减少异种移植模型中血小板的隔离。
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Chaetocin Improves Pig Cloning Efficiency by Enhancing Epigenetic Reprogramming and Autophagic Activity.鞘氨醇菌素通过增强表观遗传重编程和自噬活性提高猪克隆效率。
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PeerJ. 2019 Dec 13;7:e8143. doi: 10.7717/peerj.8143. eCollection 2019.
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Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos.决定猪体细胞核移植效率的因素:对超过200,000个重构胚胎的数据分析
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Isolation, genetic manipulation, and transplantation of canine spermatogonial stem cells: progress toward transgenesis through the male germ-line.犬精原干细胞的分离、遗传操作和移植:通过雄性生殖系进行转基因的进展。
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