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胚胎聚集调节应激条件以提高猪的发育能力。

Embryo aggregation regulates stress conditions to promote developmental competence in pigs.

作者信息

Jeong Pil-Soo, Yoon Seung-Bin, Lee Mun-Hyeong, Son Hee-Chang, Lee Hwal-Yong, Lee Sanghoon, Koo Bon-Sang, Jeong Kang-Jin, Lee Jong-Hee, Jin Yeung Bae, Song Bong-Seok, Kim Ji-Su, Kim Sun-Uk, Koo Deog-Bon, Sim Bo-Woong

机构信息

Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of Korea.

National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of Korea.

出版信息

PeerJ. 2019 Dec 13;7:e8143. doi: 10.7717/peerj.8143. eCollection 2019.

DOI:10.7717/peerj.8143
PMID:31844571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6913270/
Abstract

Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated embryos significantly improved developmental competence, including blastocyst formation rate, blastomere number, ICM/TE ratio, and cellular survival rate, compared to non-aggregated embryos. Investigation into the relationship between embryo aggregation and stress conditions revealed that mitochondrial function increased, and oxidative and endoplasmic reticulum (ER)-stress decreased compared to 1X (non-aggregated embryos) blastocysts. In addition, 3X (three-embryo aggregated) blastocysts increased the expression of pluripotency, anti-apoptosis, and implantation related genes, and decreased expression of pro-apoptosis related genes. Therefore, these findings indicate that embryo aggregation regulates stress conditions to increase developmental competence and contributes to the production of high-quality embryos and the large-scale production of transgenic and chimeric pigs.

摘要

胚胎聚集是一种在各种哺乳动物中产生具有高发育能力的囊胚以产生更多后代的有用方法，但关于其有益效果的潜在机制在很大程度上尚不清楚。在本研究中，我们研究了使用4细胞期胚胎进行胚胎聚集对猪早期胚胎发育能力的影响以及应激条件之间的关系。我们使用“孔中孔”系统进行聚集，并证实使用两个或三个胚胎进行聚集有助于获得囊胚。与未聚集的胚胎相比，聚集胚胎显著提高了发育能力，包括囊胚形成率、卵裂球数量、内细胞团/滋养外胚层比例和细胞存活率。对胚胎聚集与应激条件之间关系的研究表明，与1X（未聚集胚胎）囊胚相比，线粒体功能增强，氧化应激和内质网应激降低。此外，3X（三个胚胎聚集）囊胚增加了多能性、抗凋亡和着床相关基因的表达，并降低了促凋亡相关基因的表达。因此，这些发现表明胚胎聚集通过调节应激条件来提高发育能力，有助于生产高质量胚胎以及大规模生产转基因猪和嵌合体猪。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/ab247466f423/peerj-07-8143-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/1de9be57bc04/peerj-07-8143-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/fa439b8d8991/peerj-07-8143-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/8050793eb340/peerj-07-8143-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/38ac0f84bca1/peerj-07-8143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/7cc3a3745530/peerj-07-8143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/ab247466f423/peerj-07-8143-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/1de9be57bc04/peerj-07-8143-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/fa439b8d8991/peerj-07-8143-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/8050793eb340/peerj-07-8143-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/38ac0f84bca1/peerj-07-8143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/7cc3a3745530/peerj-07-8143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26de/6913270/ab247466f423/peerj-07-8143-g006.jpg

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