通过整体原位杂交确定基因表达模式。
Determination of gene expression patterns by whole-mount in situ hybridization.
作者信息
Kyryachenko Sergiy, Kyrylkova Kateryna, Leid Mark, Kioussi Chrissa
机构信息
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.
出版信息
Methods Mol Biol. 2012;887:15-22. doi: 10.1007/978-1-61779-860-3_2.
Whole-mount in situ hybridization (WISH) is a reliable and specific method to study three-dimensional patterns of gene expression. A labeled nucleic acid probe anneals to a complementary target sequence and is visualized and localized in the embryo. This chapter describes a sensitive method for WISH on mouse embryos using digoxigenin-labeled RNA probes. The technique can be used for the analysis of gene expression patterns during early stages of odontogenesis and in tooth explants.
全胚胎原位杂交（WISH）是一种研究基因表达三维模式的可靠且特异的方法。标记的核酸探针与互补靶序列退火，并在胚胎中可视化和定位。本章介绍了一种使用地高辛标记的RNA探针在小鼠胚胎上进行WISH的灵敏方法。该技术可用于分析牙胚发生早期阶段以及牙齿外植体中的基因表达模式。
Zotero 插件