Kyrylkova Kateryna, Kyryachenko Sergiy, Kioussi Chrissa, Leid Mark
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.
Methods Mol Biol. 2012;887:23-31. doi: 10.1007/978-1-61779-860-3_3.
In recent years, in situ RNA hybridization technique has found a widespread application in developmental biology. This method has frequently been used to determine gene expression patterns, which is a first step toward understanding of a gene function. Here, we provide a reliable and sensitive method for in situ RNA hybridization on frozen sections of mouse embryo using digoxigenin-labeled RNA probes. This technique can be used to study gene expression patterns at all stages of odontogenesis.
近年来，原位RNA杂交技术在发育生物学中得到了广泛应用。该方法经常被用于确定基因表达模式，这是理解基因功能的第一步。在此，我们提供了一种使用地高辛标记的RNA探针在小鼠胚胎冰冻切片上进行原位RNA杂交的可靠且灵敏的方法。该技术可用于研究牙发生各个阶段的基因表达模式。
