通过切片原位杂交确定基因表达模式。

Determination of gene expression patterns by in situ hybridization in sections.

作者信息

Kyrylkova Kateryna, Kyryachenko Sergiy, Kioussi Chrissa, Leid Mark

机构信息

Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.

出版信息

Methods Mol Biol. 2012;887:23-31. doi: 10.1007/978-1-61779-860-3_3.

DOI:10.1007/978-1-61779-860-3_3

PMID:22566043

Abstract

In recent years, in situ RNA hybridization technique has found a widespread application in developmental biology. This method has frequently been used to determine gene expression patterns, which is a first step toward understanding of a gene function. Here, we provide a reliable and sensitive method for in situ RNA hybridization on frozen sections of mouse embryo using digoxigenin-labeled RNA probes. This technique can be used to study gene expression patterns at all stages of odontogenesis.

摘要

近年来，原位RNA杂交技术在发育生物学中得到了广泛应用。该方法经常被用于确定基因表达模式，这是理解基因功能的第一步。在此，我们提供了一种使用地高辛标记的RNA探针在小鼠胚胎冰冻切片上进行原位RNA杂交的可靠且灵敏的方法。该技术可用于研究牙发生各个阶段的基因表达模式。