Wu Jingyi, Feng Jian Q, Wang Xiaofang
Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University College of Dentistry, Dallas, TX, USA.
Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
Methods Mol Biol. 2019;1922:163-171. doi: 10.1007/978-1-4939-9012-2_16.
In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.
原位杂交是一种常用技术，它使用反义RNA探针在组织切片上定位特定的RNA序列。这种方法可以可视化感兴趣基因在部分组织中的表达模式。在此，我们详细介绍一种使用地高辛（DIG）标记的RNA探针在小鼠石蜡切片上进行原位杂交的优化方法。
