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壳聚糖与海藻酸钙与寡聚脯氨酸和寡聚羟脯氨酸衍生物的共轭物在再生医学中的潜在应用。

Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine.

作者信息

Wasko Joanna, Fraczyk Justyna, Becht Angelika, Kaminski Zbigniew J, Grgac Sandra Flinčec, Tarbuk Anita, Kaminska Marta, Dudek Mariusz, Gliscinska Eulalia, Draczynski Zbigniew, Kolesinska Beata

机构信息

Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.

Department of Textile Chemistry and Ecology, University of Zagreb Faculty of Textile Technology, Prilaz baruna Filipovića 28a, HR-10000 Zagreb, Croatia.

出版信息

Materials (Basel). 2020 Jul 10;13(14):3079. doi: 10.3390/ma13143079.

DOI:10.3390/ma13143079
PMID:32664253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7412561/
Abstract

New materials that are as similar as possible in terms of structure and biology to the extracellular matrix (external environment) of cells are of great interest for regenerative medicine. Oligoproline and oligohydroxyproline derivatives (peptides -) are potential mimetics of collagen fragments. Peptides - have been shown to be similar to the model collagen fragment (H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH, ) in terms of both their spatial structure and biological activity. In this study, peptides - were covalently bound to nonwovens based on chitosan and calcium alginate. Incorporation of the peptides was confirmed by Fourier transform -infrared (FT-IR) and zeta potential measurements. Biological studies (cell metabolic activity by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and Live/Dead assay) proved that the obtained peptide-polysaccharide conjugates were not toxic to the endothelial cell line EA.hy 926. In many cases, the conjugates had a highly affirmative influence on cell proliferation. The results of this study show that conjugates of chitosan and calcium alginate with oligoproline and oligohydroxyproline derivatives have potential for use in regenerative medicine.

摘要

在结构和生物学特性方面与细胞外基质（外部环境）尽可能相似的新材料，对再生医学具有重大意义。寡聚脯氨酸和寡聚羟脯氨酸衍生物（肽类）是胶原蛋白片段的潜在模拟物。已证明肽类在空间结构和生物活性方面均与模型胶原蛋白片段（H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH）相似。在本研究中，肽类与基于壳聚糖和海藻酸钙的无纺布共价结合。通过傅里叶变换红外光谱（FT-IR）和zeta电位测量证实了肽的掺入。生物学研究（使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）试验和活/死检测法检测细胞代谢活性）证明，所得的肽-多糖缀合物对内皮细胞系EA.hy 926无毒。在许多情况下，缀合物对细胞增殖有高度积极的影响。本研究结果表明，壳聚糖和海藻酸钙与寡聚脯氨酸和寡聚羟脯氨酸衍生物的缀合物在再生医学中有应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/75c79293b6c1/materials-13-03079-g011a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/d45b13e3dec2/materials-13-03079-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/75c79293b6c1/materials-13-03079-g011a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/87f424fc0518/materials-13-03079-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/fc19fdf6a101/materials-13-03079-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/2a347aa83e9e/materials-13-03079-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/c0a0f1d95eb2/materials-13-03079-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/7413f5420928/materials-13-03079-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/de69b74db14a/materials-13-03079-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/bdd87a252507/materials-13-03079-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/d45b13e3dec2/materials-13-03079-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622e/7412561/75c79293b6c1/materials-13-03079-g011a.jpg

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