Puca Andrew, Russo Giuseppe, Giordano Antonio
Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology, Temple University Philadelphia, PA, USA.
Oncotarget. 2012 Apr;3(4):426-34. doi: 10.18632/oncotarget.472.
This study emphasizes the dynamical properties of mechanical loading via simulated microgravity, its effect on acute myeloid leukemia proliferation and hematopoietic stem cell (HSPC) growth and its implications in the area of tissue regeneration. Data presented illustrates that mechanical transduction changes the expression of humoral factors by facilitating paracrine/autocrine signalling, therefore modulating intracellular trafficking of tyrosine kinase receptors. Understanding mechano-transduction in the context of cell and tissue morphogenesis is the major focus of this study. The effects of external physiological stresses, such as blood flow, on several cellular subtypes seem to produce very intricate cellular responses. It is well accepted that mechanical loading plays an intrinsic and extrinsic influence on cell survival. This study shows how microgravity effects hematopoietic stem cells, and human leukemic cell proliferation and expression of its receptors that control cell survival, such as the tyrosine kinase vascular endothelial growth factor receptor-1, receptor-2 and receptor-3.
本研究强调了通过模拟微重力进行机械加载的动力学特性、其对急性髓系白血病增殖和造血干细胞（HSPC）生长的影响及其在组织再生领域的意义。所呈现的数据表明，机械转导通过促进旁分泌/自分泌信号传导来改变体液因子的表达，从而调节酪氨酸激酶受体的细胞内运输。在细胞和组织形态发生的背景下理解机械转导是本研究的主要重点。外部生理应激，如血流，对几种细胞亚型的影响似乎会产生非常复杂的细胞反应。人们普遍认为机械加载对细胞存活具有内在和外在影响。本研究展示了微重力如何影响造血干细胞、人类白血病细胞的增殖及其控制细胞存活的受体的表达，如酪氨酸激酶血管内皮生长因子受体-1、受体-2和受体-3。
