Biomicrofluidics. 2012 Jun;6(2):24113-241139. doi: 10.1063/1.3701566. Epub 2012 Apr 20.
In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate on-chip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a single-layer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics.
在这项工作中，我们展示了一种集成的、单层的、微型流式细胞仪设备，能够进行多参数粒子分析。该设备在芯片上集成了粒子聚焦和检测组件，包括基于“微流漂移”的三维（3D）流体动力学聚焦组件和一系列集成到微流结构中的光纤，以方便芯片上的检测。通过这种设计，可以同时检测单个粒子的多个光学信号（例如前向散射、侧向散射和荧光）。实验结果表明，我们的流式细胞仪芯片的性能可与体积庞大、价格昂贵的台式设备相媲美。在单个、可批量生产的微流控设备中，将芯片上的 3D 粒子聚焦与芯片上的多参数光学检测集成在一起，为用于即时临床诊断的低成本流式细胞仪芯片迈出了重要一步。
